用于作物数量性状基因座（QTL）定位研究的次级作图群体有近等基因系、导入系、染色体片段代换系等。
The secondary mapping populations (SMPs), which were used to map quantitative trait loci (QTLs), include near-isogenic lines, introgression lines, chromosome segment substitution lines et al.
该方法特别适用于精细作图群体和大型突变体库的筛选鉴定，不仅通量大、速度快，而且可以确保足够的DNA浓度和纯度。
The method is suitable for large-scale mutant bank screening as well as large mapping populations with characteristics of maintaining relatively high level of DNA purity and concentration.
主要结果如下：1。以500个F 2单株为作图群体，利用150个共显性的SSR标记，构建了覆盖玉米全基因组的分子标记连锁图。
The major results are as follows. 1. A genetic linkage map containing 150 SSR markers was constructed based on an F2 population with 500 individuals.
鉴定的微卫星分子标记为进行银鲫群体遗传学和进化遗传学研究，以及银鲫的分子标记育种和进行基因组作图提供了理想的工具。
The microsatellite markers produced by this study will be of benefit to further studies on the evolutionary genetics and provide useful tools for marker assisted selection breeding or genetic map.
鉴定的微卫星分子标记为进行银鲫群体遗传学和进化遗传学研究，以及银鲫的分子标记育种和进行基因组作图提供了理想的工具。
The microsatellite markers produced by this study will be of benefit to further studies on the evolutionary genetics and provide useful tools for marker assisted selection breeding or genetic map.
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