菌体经过超声破碎后反复洗涤包涵体，融合蛋白纯度达到70%，进一步用离子交换层析和凝胶过滤层析纯化使其纯度达95%以上。
The purity of fusion protein was about 70% after ultrasonic decomposition and washing repeatedly, and reached above 95 % after purification by ion exchange and gel-filtration chromatography.
提纯的包涵体纯度可达70%以上，用镍亲和层析方法纯化蛋白则可达到95%。
The purity of inclusion bodies was above 70% and that of protein purified by nickel affinity chromatography was 95%.
IPTG诱导表达融合蛋白，包涵体蛋白经复性后亲和层析法纯化，ELISA方法测定蛋白活性。
Fusion protein expression was induced by IPTG. After renaturation, the protein was purified by affinity chromatography and the bioactivity was examined by ELISA.
包涵体蛋白经含8m尿素的结合缓冲液溶解后，用金属亲和层析法进行纯化，获得了高纯度的目的蛋白。
After dissolution of inclusion bodies in binding buffer containing 8m Urea, the fusion protein was purified with metal affinity chromatography column, high purity protein was achieved.
方法用凝胶层析法测定116批人血白蛋白制品中多聚体的含量并对结果进行统计分析。
Method The polymers content in 116 batch of the human albumin products was determined by gelchromatography and the results were analysed by statistical method.
在金属矿地震勘探中，反射地震是一种最常用的一种方法技术，为更有效地探测与金属矿有关的局部不均匀体，我们利用了地面地震层析成像技术。
The seismic reflection is one of the seismic methods commonly used for mineral exploration. The ground seismic tomography has been adopted to detect inhomogeneous bodies related to ore deposits.
采用镍离子螯合层析法纯化变性的包涵体样品，纯度达90%以上。
The denatured inclusion bodies were purified with Ni-NTA chelate agarose and its purity can reach 90%.
薄层层析结果显示五种开口箭属植物根状茎中均含有甾体皂甙和甾体皂甙元，为开口箭属植物作为甾体药物资源提供了依据。
TLC results indicate that the rhizomes of 5 Tupistra species contain steroidal saponin and sapogenin, it provides evidence for Tupistra as medicine resources.
薄层层析结果显示五种开口箭属植物根状茎中均含有甾体皂甙和甾体皂甙元，为开口箭属植物作为甾体药物资源提供了依据。
TLC results indicate that the rhizomes of 5 Tupistra species contain steroidal saponin and sapogenin, it provides evidence for Tupistra as medicine resources.
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