目的检测犬贾第虫病毒介导的锤头状核酶对犬贾第虫滋养体核仁功能性蛋白krr1基因体外转录体切割效率。
Objective to detect the cleavage activity of Giardia Canis virus (GCV) transfer vector-mediated hammerhead ribozyme for KRR1 in vitro transcript.
实验选用3-磷酸甘油醛脱氢酶基因(GAPDH)作为内参照,体外转录的RNA和阳性质粒作为两种标准品。
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene was served as the internal reference, and RNA transcribed in vitro and positive plasmid were served as the standards in this experiment.
实验选用3-磷酸甘油醛脱氢酶基因(GAPDH)作为内参照,体外转录的RNA和阳性质粒作为两种标准品。
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene was served as the internal reference, and RNA transcribed in vitro and positive plasmid were served as the standards in this experiment.
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