我们首先得做一个体外细胞培养实验，在动物模型中证明它的存在，然后开发出一种能影响突变基因产物的分子或蛋白质。
We have to do in-vitro cell culture experiments first, prove it in an animal model, and then develop a molecule or protein which will affect the mutated gene product.
方法采用体外细胞培养计数法。
Methods: In vitro culture cell number count assay was adopted.
方法采用体外细胞培养计数法，结果表明，该多糖对胃癌有显著性抑制作用。
Methods in vitro culture cell number count was adopted. Results AGP significantly inhibited the gastric cancer cell.
资料选择：选择共轭亚油酸抗癌的动物实验及体外细胞培养研究，作用机制研究以及人工合成方法等方面的相关文献45篇。
STUDY SELECTION: Totally 45 literatures were selected about anticarcinogenic effect of CLA using animal experiments, cell culture in vitro, mechanisms and synthetical methods of CLA.
通过体外心肌细胞培养试验和动物实验，探讨了砷对心脏某些生化功能的影响。
The effects of sodium arsenite on certain Biochemical Function in heart of mice were explored by myocardial cell culture in vitro and whole animal experiment.
方法应用体外细胞培养技术观察MC3T3 - E1成骨细胞株在不同处理的牙骨质表面的生长状况。
Methods Cell culture technique in vitro had been used to study the growth condition of MC3T3-E1 osteoblast line on the surface of cementum treated with various methods.
改进体外成肌细胞培养方法。
方法用酶消化、分离鸡胚关节软骨细胞，体外单层培养，收集细胞培养液。
Methods Chick embryo joint chondrocytes digested and isolated by enzyme were grown monolayer culture in vitro. Chondrocyte culture media was collected.
方法：体外细胞培养，MTT （噻唑蓝）比色法，材料直接接触法。
METHODS:Methods of in vitro cell culture model of human primary PDLC, tetrazolium bromide reduction (MTT) assay and direct contact were used.
由于缺乏有效的体外细胞培养体系和动物模型，人博卡病毒与人类呼吸道或胃肠道疾病的临床关联尚且未知。
Since no in vitro cell culture systems and animal models are available, whatever role HBoVs plays in respiratory infections and gastrointestinal illness remains uncertain.
目的：探讨利用旋转式细胞培养系统（RCCS）在体外快速构建组织工程骨的可行性。
Objective: To explore the possibility of rapid construction of tissue engineered bone in vitro by RCCS.
目前，HIV - 1黏膜感染机制的研究主要依赖于体外细胞培养和灵长类动物模型。
Currently, study of the mechanism of HIV-1 mucosal infection majorly depends on in vitro cell culture systems and non-human primate animal models.
体外肝细胞模型(主要是原代肝细胞培养)作为一种强有力的体外研究体系被广泛应用于化学异物的肝毒性研究。
In vitro liver cell models (mainly primary hepatocyte cultures), served as the most powerful in vitro research system are widely used for the study of hepatotoxicity of xenobiotics.
方法采用常规细胞培养法进行骨膜细胞体外培养，并观察其形态特征及超微结构。
Methods To observe the morphological characteristic of human periosteal cells cultivated by the conventional method in vitro under optics and electronic microscope.
目的：动物细胞培养基是体外细胞培养的重要因素，能够影响细胞生长。
OBJECTIVE: Animal cell culture medium is an important factor of cell culture in vitro, which can influence the cell growth.
方法采用体外新生大鼠皮层神经细胞培养法观察微量元素碘对神经细胞表达nse、NGF和IGFR蛋白的影响。
Methods We use the model of cortical neuronal culture in neonatal rats in vitro and observe the NSE, NGF and IGFR expressions in cultured neurons by immunohistochemical method.
方法体外细胞培养，H2O2处理PC 12细胞复制氧化损伤模型。
Method PC12 cell was exposed to H2O2 in vitro to establish oxidative injury model.
方法利用体外细胞培养模型，应用MTT法分析姜黄素衍生物对不同肿瘤细胞生长的影响。
METHODS Based on cell culture model in vitro, MTT test was used to study the inhibitory effect of curcumin derivatives on tumor cell lines.
体外肝细胞培养是生物制药和人工肝支持系统的技术基础。
Cultured cells are the basic element of biologic pharmacy and artificial liver system.
运用艾灸血清和小鼠胸腺细胞瘤(EL - 4)进行细胞培养，观察艾灸血清在体外对肿瘤细胞形态、细胞周期及DNA断点的影响。
By culture EL-4 tumor cells with Murine Moxibustion Serum (MS), influence of MS to cell structure, cell cycle and breaking point of DNA was studied.
少突胶质前体细胞培养基是专门为少突胶质前体细胞体外分化设计的最适宜的培养基。
Oligodendrocyte precursor cell differentiation medium is a complete medium designed for the differentiation of oligodendrocyte precursor cells in vitro.
方法：采用细胞培养的方法建立稳定传代的胰腺癌hs 766t细胞系，体外培养人脐带静脉内皮细胞。
Methods: We built the pancreatic cancer cell group HS 766t and human endothelium cell in the umbilical vein by the way of cell culture in vitro.
方法：采用经具核梭杆菌LPS刺激的单核细胞培养上清作用于体外培养的人牙髓细胞，通过OD值测定，观察单核细胞培养上清对人牙髓成纤维细胞碱性磷酸酶（ALP）活性的影响。
Methods:The culture supernatant of monocytes stimulated with Fn LPS was applied to human dental pulp cell in vitro, and activity of ALP in human dental pulp fibroblast was monitored by OD test.
材料和方法：采用酶辅助的显微分离法分离新生牛眼ipe细胞，酶消化法分离角膜内皮细胞，进行体外细胞培养。
Material and Methods: From bovine eyes IPE cells were isolated with enzyme-assisted microdissection, corneal endothelium cells with enzyme digestion and then were cultured in vitro.
方法：利用体外细胞培养、MTT、NBT染色及吞噬功能测定等方法，观察在不同浓度金龙胶囊作用下人早幼粒白血病细胞系(HL - 60)的细胞形态和功能的变化。
Methods: in vitro cell culture, MTT titration, NBT reduction and phagocytosis determination were employed to study the effects of JLC on HL-60 cells.
体外细胞培养及动物实验证明，促红细胞生成素对脑缺血损伤具有神经保护和治疗作用。
It has been proved that erythropoietin has neuroprotective effect and therapeutic action on cerebral ischemia in vivo and in vitro.
从而进一步求证成骨细胞在体外造血干祖细胞培养体系中的支持作用，并试图从不同的角度认识成骨细胞与造血的相互关系。
Furthermore, reciprocal cooperation between HSCs and osteoblasts play a profound and central role in the establishment and maintenance of the stem cell niche in the bone marrow.
内皮细胞培养基是专门为正常人类微血管内皮细胞体外培养设计的最适于其生长的完全培养基。
Smooth muscle cell medium (SMCM) is a complete medium designed for optimal growth of normal human smooth muscle cells in vitro.
结论：旋转式细胞培养系统是体外快速构建组织工程化骨的理想方法。
Conclusion: RCCS may be an ideal method for rapid construction of tissue engineered bone in vitro.
我们应用体外细胞培养的方法，已证实蝎毒素对大肠癌细胞具有显著的抑杀作用。
It was found that scorpion venom had notable inhibition of colorectal cancer cells by in vitro cell culture.
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