背景与目的：建立一种稳定的人卵母细胞体外培养成熟技术。
BACKGROUND AIM: To establish a stable in vitro culture technique of human oocytes.
研究结果证明，牛卵巢卵母细胞可作超低温冷冻长期保存，解冻后可以体外培养成熟。
These results indicate that bovine follicular oocytes can be frozen for long term preservation in liquid nitrogen and mature in vitro after thawing.
体外培养成熟的卵母细胞，吸卵管反复吹打去除颗粒细胞，电子显微镜下观察其部分超微结构。
After the completion of culture of oocytes for IVM, cumulus cells were removed. Ultrastructure was studied under electron microscope.
结果表明海狸鼠卵母细胞与其它啮齿动物的卵母细胞一样，能够在体外培养成熟，完成第一次减数分裂，排出第一极体。
The results indicated that nutria oocytes, like other rodent oocytes, can be matured in vitro, complete first meiotic division and discharge first polar body.
结果表明海狸鼠卵母细胞与其它啮齿动物的卵母细胞一样，能够在体外培养成熟，完成第一次减数分裂，排出第一极体。
The results indicated that nutria oocytes, like other rodent oocytes, can be matured in vitro, complete first meiotic division and discharge first polar body.
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