方法体外分离培养人成骨细胞。
Methods The osteoblasts were isolated from human fetal calvaria.
方法体外分离、培养和鉴定BMSC。
Methods Human BMSCs were isolated, incubated and identified.
目的探讨建立人皮肤汗腺细胞体外分离及纯化培养的技术。
Objective To investigate the method of isolation and purification of epithelial cells of human eccrine sweat gland in vitro.
目的：建立免疫磁珠法体外分离纯化人牙周膜干细胞的方法。
Objective: To isolate and identify the periodontal ligament stem cells from human molars.
探讨动态力学应变对体外分离培养的人骨膜细胞的生物学效应。
The objective of this program is to investigate the biological effect of dynamic strain on human periosteal cells in vitro.
目的：为了建立免疫磁珠法体外分离提纯骨髓造血干细胞的方法。
Objective: to isolate and purify murine hematopoietic stem cell using the immunomagnetic microbeads.
目的对兔骨髓间质干细胞进行体外分离培养并观察其生物学特性。
Objective To isolate and culture the rabbit mesenchymal stem cells, and explore the biological features in vitro.
目的：体外分离培养并鉴定人外周血树突状细胞，并观察其抗原呈递功能。
AIM: To isolate, culture and identify in vitro of dendritic cells (DCs) from human peripheral blood, and observe the function of antigen presentation.
研究人胎肝干细胞体外分离纯化方法，并对其进行初步鉴定及生物学特性分析。
To explore the methods of isolation and purification of human fetal liver stem cells in vitro and analyze the bionomics.
目的：探讨体外分离培养人脐带间充质干细胞的方法，并对其进行生物学鉴定。
Objective:To explore the method of isolate and culture of human umbilical cord mesenchymal stem cells (hUCMSCs) in vitro, and identify their biological characteristic.
结论：脐血成分复杂，在体外分离培养可以获得形态各异的脐血间充质样细胞。
Conclusion: Cord blood mesenchymal-like cells can be cultured in vitro, and mesenchymal-like cells with varied morphology can be obtained.
采用微吸管吸取技术，我们体外分离出大鼠的味蕾，将其培养在LAPS芯片上。
Using micropipette, the taste buds were successfully isolated from rat tongue epithelium and then placed on LAPS chip.
目的：探讨体外分离、培养和扩增大鼠骨髓来源树突状细胞(DC)的有效方法。
Objective: To study the effective method of isolation, culture and proliferation of rat bone marrow-derived dendritic cells (DC) in vitro.
目的：采用两种改良方法体外分离培养脐血间充质干细胞，并观察其成骨分化能力。
AIM: to culture in vitro umbilical cord blood mesenchymal stem cells (UCB-MSCs) by two modified methods and to observe their osteogenic differentiation potential.
目的：探讨人脂肪源干细胞在体外分离培养扩增的方法及向成骨细胞诱导分化的能力。
OBJECTIVE: to establish a method for the isolation and culture of human ADSCs and to explore the ability of osteogenic differentiation.
方法：1体外分离培养大鼠骨骼肌卫星细胞，免疫组化鉴定骨骼肌卫星细胞并检测其纯度。
Methods: 1. Primary rat skeletal muscle satellite cell was cultivated in vitro. The cultured cells were identified with cellular immunochemical stain.
目的：体外分离、培养人乳牙牙髓干细胞，分析其生物学特征及向成骨细胞诱导分化的能力。
PURPOSE: to isolate and culture dental pulp stem cells from human deciduous teeth, then to analyze their biological characteristics and potential to be induced to osteoblasts.
对两例原发性肾癌患者手术切除肿瘤组织中肿瘤浸润性淋巴细胞（TIL）进行了体外分离与培养。
Tumor-infiltrating lymphocytes (TIL) isolated from two renal cell carcinoma were cultured in vitro in the presence of rh IL-2.
结论局部应用体外分离培养扩增的单核细胞对神经再生修复有一定的促进作用，其作用优于淋巴细胞。
Conclusion After being purified and amplified in vitro and auto-transferred around the nerve, the monocyte positive effects on facial nerve repair and regeneration are better than lymphocytes.
目的：比较人胎盘源间充质干细胞和人骨髓源间充质干细胞体外分离培养、扩增及生物学性状的差异。
OBJECTIVE: To compare the differences in isolation and culture in vitro, amplification and differences in biological characteristics between PMSCs and BMSCs.
目的：拟建立一套体外分离培养及大量扩增兔骨髓间充质干细胞的方法，并对细胞生物学特性进行观察。
OBJECTIVE: To establish a method for in vitro isolation and cultivation of BMSCs from rabbits and to study their biological characteristics.
选用摘除双侧卵巢的山羊颅盖骨，体外分离培养成骨细胞，并对分离的成骨细胞做多方面生物学特性鉴定。
Osteoblasts were isolated from the frontal parietal bone of ovariectomized goat and were confirmed by testing for multiple biological features.
目的：观察大鼠骨组织来源成骨细胞的体外分离培养的条件及生长特征，为骨组织工程学实验研究奠定基础。
Objective: to observe culture condition and growth characteristics of osteoblasts in vitro, which were developed from bone tissue of mice, contributing to the bone tissue engineering experiment.
结果与结论：体外分离培养的脂肪源干细胞生长稳定，扩增速度快。流式细胞仪检测结果显示其高表达干细胞相关抗原。
RESULTS and CONCLUSION: ADSCs could be expanded steadily in vitro and flow cytometry revealed high expression of stem cell-associated markers.
由于在体环境异常复杂，细胞生理或病理现象的研究仍依赖于细胞体外分离和培养技术，因此，构建细胞体外加载实验系统是目前研究细胞的力学响应机制的主要途径。
Because of the staggering complexity of the in vivo environment, studying cellular responses to mechanical stress and strain has relied heavily on the use of in vitro mechanical loading systems.
方法：体外分离培养大鼠骨髓间充质干细胞，传代后通过形态学和流式细胞仪检测细胞表面标志物cd14, CD 44, CD 9,CD 34, CD 45表达。
METHODS: The rat BMSCs were separated and cultured in vitro. Following passage, expression of CD14, CD44, CD9, CD34 and CD45 was detected by morphology and flow cytometry.
方法用酶消化、分离鸡胚关节软骨细胞，体外单层培养，收集细胞培养液。
Methods Chick embryo joint chondrocytes digested and isolated by enzyme were grown monolayer culture in vitro. Chondrocyte culture media was collected.
加的夫公共健康实验室从一株分离株中克隆了病毒的拷贝，让我们去识别能引起体外病毒不稳定的基因。
Cloning a copy of the virus from a strain isolated by Cardiff Public Health Laboratories has enabled us to identify the genes causing the instability of the virus outside the body.
加的夫公共健康实验室从一株分离株中克隆了病毒的拷贝，让我们去识别能引起体外病毒不稳定的基因。
Cloning a copy of the virus from a strain isolated by Cardiff Public Health Laboratories has enabled us to identify the genes causing the instability of the virus outside the body.
应用推荐