冷冻的大脑切片被保存在低温条件下，直到进行进一步切片处理并最终用RNA加以探测，以寻找特定的基因表达。
Frozen brain slabs are kept at cryogenic temperatures until they are further sliced and eventually probed with RNA to look for specific gene expression.
通过聚丙烯酰胺凝胶电泳技术与石蜡切片等技术研究了低温解除龙牙百合休眠过程中糖类物质的转化。
The transform of glucide in the course of the dormancy by low temperature was studied in the paper in paraffin section and polyacrylamide gel electrophoresis.
方法：采用改进的甲基丙稀酸甲酯低温塑料包埋法包埋SD大鼠胫骨近端，制备不脱钙的骨组织切片。
Methods The proximal tibiae of SD rats were embedded with the modified methylmethacrylate low-temperature embedding method and then made into undecalcified bone sections.
方法经过灌流固定的CNS组织低温冰箱速冻后连续冰冻切片和改良的苏木精染色方法。
Methods After perfused fixation and quick freezing, the tissues of whole CNS were cut into serial sections with cryostat followed by hematoxylin stain.
目的：探讨在低温塑料包埋制备的不脱钙骨切片上运用免疫组化和原位杂交技术的可行性。
Objective To investigate the feasibility of applying immunohistochemistry (IH) and in situ hybridization (ISH) technique to the undecalcified bone sections.
结论低温骤冷法是这三种防冰晶法中最利于保存切片的方法。
Conclusion the liquid nitrogen rapid freeze method is the best anti-ice crystal mation method for section preservation.
结论低温塑料包埋方法制备的人类不脱钙骨组织切片可同时进行免疫组化和原位杂交的检测观察。
Conclusions The undecalcified bone sections of human made in the method can satisfy the need of IH and ISH.
结论低温塑料包埋方法制备的人类不脱钙骨组织切片可同时进行免疫组化和原位杂交的检测观察。
Conclusions The undecalcified bone sections of human made in the method can satisfy the need of IH and ISH.
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