从人胚胎生殖嵴、肠系膜中消化分离的原始生殖细胞，将其接种在人子宫内膜成纤维细胞饲养层上传代培养。
PGC from genital ridge and mesenterium of human embryo was incubated on fibroblast feeder layers for subculture.
目的探索人心房肌细胞的原代及传代培养方法。
Objective To explore a method for primary culture and subculture of human atrial myocardial cells.
结果原代培养的细胞ALP染色阳性区域可以达90%以上，传代培养后细胞阳性率为100%。
Results the area positive for ALP staining is no less than 90% in the primary culture and almost 100% in the subculture.
背景：人胚胎干细胞传代培养的关键是抑制其自发分化、保证细胞的全能性。
BACKGROUND: Key point for subculture of human embryonic stem cells is to inhibit spontaneous differentiation and make sure totipotency of cells.
细胞传代培养和冻存6个月，7td1MC 5细胞对IL - 6的反应性和依赖性均无明显变化。
No obvious variation of reactivity and dependence of 7td1 MC5 cell on IL-6 was observed after 6 months Subculture and Cryostorage.
传代培养细胞可在1 ~2周汇合成片。
在DMEM培养基中进行了原代及传代培养，对其中的成纤维细胞进行分离纯化。
The isolated cells were cultured in DMEM medium and PDL fibroblasts were purified in subculture.
在DMEM培养基中进行了原代及传代培养，对其中的成纤维细胞进行分离纯化。
The isolated cells were cultured in DMEM medium and PDL fibroblasts were purified in subculture.
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