以120份疑似猪瘟病料为试验材料，使用RT-PCR核酸检测与ELISA抗原检测2种方法，分别用这2种方法检测疑似病料，并且比较这2种方法在猪瘟病料检测中的实际差别。
In order to analyze the differences between RT-PCR and ELISA antigen detection methods in classical swine fever virus detection, 120 clinical samples were detected by the two methods.
方法用盐提取法提取人群中白细胞的DNA，以聚合酶链反应、限制性内切核酸酶检测-4533G/A多态性。
Methods DNAs were extracted from the white blood cells of people in Hainan by salt-out method. Polymerase chain reaction and restriction endonuclease was used to determine the 4533G/A polymorphism.
该方法可 以应用于生物检测的许多领域，例如：核酸杂交检测、核酸损伤检测和化学物质基因毒性的检测。
The invention can be used in biological detection, as nucleic acid cross detection, nucleic hurt detection and chemical material gene toxicity detection.
该方法可 以应用于生物检测的许多领域，例如：核酸杂交检测、核酸损伤检测和化学物质基因毒性的检测。
The invention can be used in biological detection, as nucleic acid cross detection, nucleic hurt detection and chemical material gene toxicity detection.
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