方法：取第5代细胞进行免疫组化，检测其免疫表型。
Methods: carry on immunohistochemistry for the 5th generation cells to detect the immune phenotype.
方法原代培养角膜基质细胞，选取第4代细胞进行实验。
Methods Primary human corneal fibroblasts of passages 4 were used in this research.
至第7代细胞活力呈明显下降趋势，细胞成活率仅为78%。
However, the activity of the seventh passage of cells was significantly decreased, and the survival rate was only 78%.
培养的第2～3代细胞适用于缺氧实验和药物作用机制的研究。
Cultured pulmonary trunk wall cells at the 2nd to 3rd passage were applicable to investigations of hypoxic experiments and efficiency of drugs.
结果表明在细胞生长对数期继代比静止期继代细胞增长率提高1.6倍，而紫杉醇产量差异不大。
The results indicated that the growth rate of cells subcultured at exponential stage was 1.6 times of that at stationary stage and there was no significant difference in the taxol content.
其后，作者通过对CD 8 +T细胞不对称细胞分裂后的第一代两个子代细胞研究，证明了与T细胞命运相关的分子遗传性。
The authors next examined the inheritance of T-cell-fate-associated molecules by the first two daughter cells following asymmetrical cell division of CD8 + t cells.
细胞群体倍增时间由第7代起显著延长（P<0.01）；MTT比色法的结果亦显示，第7代细胞的增殖能力开始大幅下降（P<0.01）。
Results Population doubling time of corneal stromal cells prolonged significantly since passage 7. MTT assay also showed cell viability of passage 7 decreased to 60% of passgage 1 (P< 0. 01 ).
外因遗传是一种可以传给下一代细胞的基因调控过程，最常见的方式是通过甲基化作用来完成，该作用可以让甲基（由一个碳原子和三个氢原子组成）附着于腺嘌呤或胞嘧啶上。
Epigenetics is a type of gene regulation that can be passed from a cell to its daughters. The most common mechanism is methylation.
观察二月兰一代杂交种子的细胞分裂。
Observe the meiosis on the F1 hybrid of Orychophragmus violaceus.
尽管这通常注定某个细胞的基因无法传给下一代，但却使得联合形成生物体可以间接地将基因成功遗传给下一代。
Though this often doomed an individual cell's own genes, it gave rise to a co-operative organism that successfully passed those genes on indirectly.
这些变异对于细胞向卤代基的转化的影响将是后续研究的课题。
The contribution of these mutations to the adaptation of the cells towards the halogenated base will be the subject of follow-up studies.
这一方法可能会引发“新一代”抗癌药物的诞生，该药物将可以用于阻止肿瘤细胞通过“转移”的过程向身体其他部位扩散。
The discovery might lead to a new generation of anti-cancer drugs that prevent tumour cells from spreading to other parts of the body through a process called metastasis.
这些酶(在1970年代由史密斯发现，因此赢得了诺贝尔奖)可以潜伏在已经没有DNA的细胞尸体中，在合成基因组起作用前切断它们。
These enzymes (discovered in the 1970s by Dr Smith, in work that won him a Nobel prize) would lurk in the DNA-free cadaver and cut up the synthetic genome before it was able to do its stuff.
上世纪90年代，这些细胞在猕猴身上被发现，当时研究人员在动物执行不同任务的同时记录植入动物大脑中的微电极示数。
Discovered in macaques in the 1990s, these cells were spotted when researchers made recordings from microelectrodes placed in the animals' brains as they performed various tasks.
上世纪80年代他们展示了端粒(染色体两端的特殊帽子)如何保护细胞中的DNA和基因免遭降解。
In the 1980s they showed how telomeres, distinctive caps on the ends of chromosomes, protect a living cell's DNA and genes from degradation.
早在上世纪九十年代，伯克博士就发现笑会增加癌症病人天然杀伤细胞(也叫抗癌细胞)的数目，天然杀伤细胞是身体对抗肿瘤的办法。
In the mid-1990s, Dr Berk found that laughter increases the number of natural killer cells in cancer patients. Natural killer cells are the body's way of fighting tumours.
20世纪70年代，托德·亨尼西(ToddHennessey)声称，单细胞池塘居民草履虫，可以达到实验室要求的条件。
In the 1970s, Todd Hennessey claimed that paramecia, the single-celled pond dweller, could be conditioned in the lab.
细胞生物学家Herman，自1980年代初期起就对周细胞进行研究。
Herman, a cell biologist, has been studying pericytes since the early 1980s.
上世纪九十年代，颠覆了数十年经验教条的一项发现表明，哺乳动物，包括人类，终其一生都在产生新的神经细胞。
In the 1990s, decades of dogma were overturned by the discovery that mammals, including people, make new neurons throughout their lives.
英国生化学家AnthonyAllison在20世纪50年代观察到非洲人携带单拷贝的基因突变引起的镰状细胞性贫血保护了他们受到疟疾的侵扰。
In the 1950s Anthony Allison, a British biochemist, observed that Africans who carry a single copy of the genetic mutation that causes sickle-cell anaemia are protected against malaria.
然而就像兰蒂克女士所述，很少有门外汉知道早在20世纪60年代就有科学家首次将不同物种的细胞融合在一起。
Yet few laymen realise that scientists first fused cells from different species as far back as the 1960s, as Ms Landecker describes.
70年代，另一位年轻的科学家，迈克尔卡普兰发现在大鼠脑中存在神经再生现象（即神经元细胞新生）。
Then, in the 1970's, another young scientist, Michael Kaplan discovered neurogenesis (the birth of new neurons) in the brains of rats.
研究人员在70年代早期取得了很大进展，他们发现导致癌症的癌基因在正常细胞里是不活跃的。
The researchers made great progress in the early 1970s, when they discovered that oncogenes, which are cancer-causing genes, are inactive in normal cells.
它隐含在她的系列装置艺术卓品“细胞”之中，在该1990年代作品中，透过半开的门或肮脏的小窗户，可以看到一些小而荒凉的房间。
It lay behind the "Cells", her series of installations in the early 1990s in which small, bleak rooms were viewed through half-opened doors or dirty Windows.
结果表明，体外培养人胚肺二倍体成纤维细胞成活至52代，而含0。2%黄芪制取液的培养基使细胞成活至77代。
The results showed that heldf cultured in vitro lived up to52generations while the culture medium containing0.2% amb extract made the cells live up to77genera-tions.
新一代的光学手段是显现能够提供内部细胞工作的详细图片。
A new generation of optical instruments is emerging that can provide detailed imaging of the inner workings of cells.
他们在20世纪80年代晚期发现，尽管一串线性氨基酸可以在试管中折叠成特定的三维形状，但蛋白质自身不能在细胞内完成这一过程。
In the late 1980s, they discovered that although a linear string of amino acids can fold into its proper three-dimensional shape in a test tube, the protein cannot do this on its own within a cell.
他们在20世纪80年代晚期发现，尽管一串线性氨基酸可以在试管中折叠成特定的三维形状，但蛋白质自身不能在细胞内完成这一过程。
In the late 1980s, they discovered that although a linear string of amino acids can fold into its proper three-dimensional shape in a test tube, the protein cannot do this on its own within a cell.
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