方法：1。 人骨髓间充质干细胞的分离与培养。
目的：体外观察人骨髓间充质干细胞对再生障碍性贫血患者T细胞增殖的影响。
OBJECTIVE: To explore effects of human BMSCs on t cell proliferation in patients with aplastic anemia in vitro.
方法取人骨髓间充质干细胞，使其附着于冻干松质骨和羟基磷灰石，并植入裸鼠皮下。
Methods the human bone marrow mesenchymal stem cells, which adhered to freeze dried cancellous and hydroxyapatite, were implanted in the nude mice subcutaneously.
将人骨髓间充质干细胞种植于CPC - PLGA复合物上并体外培养，然后进行扫描电镜观察。
Human bone marrow mesenchymal sten cells (BMSCs) were seeded into CPC-PLGA polymer and cultured in vitro, then detected by SEM.
结论：经比较羟乙基淀粉沉淀法明显优于其他分离方法，在人骨髓间充质干细胞的培养过程中建议使用此种物理沉降方法分离骨髓有核细胞。
This physical sedimentation method is a very good method that can be used during the culture of MSCs to separate nucleated cells in bone marrow.
人脐带和胎盘来源的MSC取材容易，较骨髓间充质干细胞有更广泛的应用前景。
Human umbilical cord-derived and human placenta-derived MSCs are easily available, which have more widespread application prospect than bone marrow-derived MSCs.
来自人脂肪组织的间充质干细胞与骨髓间充质干细胞是一类具有非常相似表型的细胞群。
MSC from human bone marrow and adipose tissue represent very similar cell populations with comparable phenotypes.
间充质干细胞（MSC）起源于中胚层，主要位于成体骨髓和人胎儿脐血，与造血干细胞共存。
Mesenchymal stem cell (MSC), originating from mesoblast, resides mainly in post-natal bone marrow as well as human umbilical cord blood, and coexists with hematopoietic stem cell (HSC).
目的：比较人胎盘源间充质干细胞和人骨髓源间充质干细胞体外分离培养、扩增及生物学性状的差异。
OBJECTIVE: To compare the differences in isolation and culture in vitro, amplification and differences in biological characteristics between PMSCs and BMSCs.
目的：比较人胎盘源间充质干细胞和人骨髓源间充质干细胞体外分离培养、扩增及生物学性状的差异。
OBJECTIVE: To compare the differences in isolation and culture in vitro, amplification and differences in biological characteristics between PMSCs and BMSCs.
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