目的：观察分期人自体角膜缘干细胞移植治疗中重度眼部碱烧伤的临床效果。
AIM: to observe clinical effects of corneal limbus stem cell autograft in treating moderate-severe ocular alkaline burn by stages.
目的观察人羊膜移植联合自角膜缘干细胞移植治疗中重度眼部碱烧伤的临床效果。
ObjectiveTo observe the clinical effects of amniotic membrane transplantation and combination stem cell of self-limbal graft for medium and severe ocular alkaline burn.
经反复传代，初步建立了人胚胎角膜上皮细胞系，并对其生物学特性进行了研究。
After human embryo corneal epithelial cell line was established through many cell generations, its biological character was researched.
因此，人胚胎角膜上皮细胞适合于建立相对稳定的细胞系。
Thus, the human embryo corneal epithelial cells was fit for establishing a relative stable cell line.
方法采用低钙培养法对人角膜缘基底层细胞组织块进行培养。
Methods Limbal button with basal cells was cultured in low calcium medium.
方法利用体外培养的单层近融合期人RPE细胞，采用棉签和角膜移植用环钻做圆形细胞刮伤区，建立体外RPE细胞损伤模型。
Methods Cultured monolayer-confluent human RPE cells were scraped with a trephine and a cotton stick, and set up the injured model of RPE cells with round scraped area.
兔角膜上皮和内皮细胞组织块原代培养，消化接种于人胚肺饲细胞瓶传代培养。
After primary culture, rabbit corneal epithelium and endothelium were digested and inoculated in bottle having hFLP feeder cells for serial passage.
目的：用体外培养的角膜缘上皮细胞联合人羊膜的方法，行自体移植治疗角膜缘干细胞完全缺乏的兔眼。
Objective: to study in vitro cultivated limbal epithelial cells autograft with amniotic membrane grafts for total limbal stem cell deficiency of rabbit eyes.
目的通过检测人角膜缘组织中P 63、K3蛋白表达的情况，探讨P 63蛋白作为角膜缘干细胞阳性标记的可能。
Purpose to detect P63, K3 expression in human limbus, to approach the possibility of P63 acted as the marker of human limbal stem cells.
目的探讨培养的异体人角膜缘干细胞移植治疗眼表疾病的方法和疗效。
Objective to investigate techniques and effects of cultivated limbal stem cells allograft to treat ocular surface disorders.
方法应用消化培养法对人胎儿角膜缘上皮组织进行体外培养并观察记录培养细胞的生长特性；
Methods Human fetal limbal epithelial tissue was cultured in vitro by enzymatic digestion culture method and the morphological characteristics of cultured cells were recorded.
方法：采用自体或异体角膜缘干细胞联合保存人羊膜移植治疗重症化学伤、眼球粘连、复发性胬肉。
Method:Autologous or allosome limbal stem cells and human amnion were used in 48 patients with severe chemical injury, eyeball adhere, recurrent pterygium.
方法：采用自体或异体角膜缘干细胞联合保存人羊膜移植治疗重症化学伤、眼球粘连、复发性胬肉。
Method:Autologous or allosome limbal stem cells and human amnion were used in 48 patients with severe chemical injury, eyeball adhere, recurrent pterygium.
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