方法从培养的人胚肺成纤维细胞(WI- 38)中提取总rna，经rt -PCR获得人破骨细胞抑制因子基因。
METHODS Total RNA was extracted from human embryonic lung fibroblast cells (WI 38) and the whole length gene of human OPG was obtained by RT PCR.
研究发现，SO_2及其衍生物可引起人外周血淋巴细胞、小鼠骨髓细胞、中国仓鼠肺成纤维细胞等哺乳动物细胞的染色体畸变、姊妹染色单体交换及微核的形成。
Several studies have shown that SO2 and its derivatives could induce chromosomal aberrations, sister chromatid exchanges and micronuclei in human blood lymphocyte, bone marrow cell in mice and CHL.
同时以端粒酶阳性的永生化人胚肾成纤维细胞HEK293作为阳性对照，以端粒酶阴性的人胚肺成纤维细胞HELF作为阴性对照。
Immortalized human embryonic kidney cell line HEK293 and the human embryonic lung fibroblasts HELF were used as positive control and negative control, respectively.
目的：研究依他酸（EA）和卡西霉素（Cal）对人胚肺成纤维细胞脱氧核糖核酸和胶原合成的影响。
AIM: To study the effects of egtazic acid (EA) and calcimycin (Cal) on the synthesis of DNA and collagen in cultured human lung fibroblasts (HLF).
目的：研究依他酸（EA）和卡西霉素（Cal）对人胚肺成纤维细胞脱氧核糖核酸和胶原合成的影响。
AIM: To study the effects of egtazic acid (EA) and calcimycin (Cal) on the synthesis of DNA and collagen in cultured human lung fibroblasts (HLF).
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