结果人羊膜上皮细胞可以在体外成功的培养传代，体外可连续传8 ~10代。
Results Human amniotic epithelial cells were successfully cultured and passaged in vitro, and could be passaged successively for 8-10 times.
结论人羊膜上皮细胞在体外可成功进行原代和传代培养，体外培养的人羊膜上皮细胞在一定时间内可维持增殖能力。
Conclusion Human amniotic epithelial cells can be successfully primary cultured and passaged in vitro, and the cells can proliferate for a certain period.
目的：用体外培养的角膜缘上皮细胞联合人羊膜的方法，行自体移植治疗角膜缘干细胞完全缺乏的兔眼。
Objective: to study in vitro cultivated limbal epithelial cells autograft with amniotic membrane grafts for total limbal stem cell deficiency of rabbit eyes.
目的：用体外培养的角膜缘上皮细胞联合人羊膜的方法，行自体移植治疗角膜缘干细胞完全缺乏的兔眼。
Objective: to study in vitro cultivated limbal epithelial cells autograft with amniotic membrane grafts for total limbal stem cell deficiency of rabbit eyes.
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