方法提取人外周血单个核细胞总RNA，反转录聚合酶链反应（RT-PCR）扩增目的基因；
Methods RNA from human peripheral blood mononuclear cells were extracted and target genes were amplified by RT-PCR.
目的检测白细胞介素-2（IL-2）联合IL-12诱导人外周血单个核细胞（PBMC）体外抗肿瘤作用。
Objective To examine the in vitro antitumor activity of normal human peripheral blood mono-nuclear cells(PBMC) induced by interleukin-2(IL-2) and IL-12.
方法建立人脑胶质瘤和转输人外周血单个核细胞于SCID小鼠体的人肿瘤人淋巴细胞的嵌合模型，并用SEC治疗之。
Methods Human lymphocytes were transplanted to human glioma-bearing SCID mice to establish the chimeric model of human tumor and lymphocytes. SEC was applied for tumor treatment.
结论以人外周血单个核细胞基因组dna为模板，扩增人CD 14基因的方法是一种获取CD 14基因简捷、有效的方法。
Conclusion It is a simple and effective method to obtain human CD14 gene from genome DNA of human mononuclear cell in peripheral blood.
探讨脱氧雪腐镰刀菌烯醇（DON）对人外周血单个核细胞参与抗原呈递的低分子量蛋白酶体-2（LMP-2）表达的影响。
To explore the effects of deoxynivalenol (DON) on low molecular weight polypeptide-2 (LMP- 2) expression of human peripheral blood mononuclear cells.
应用抗CD3单克隆抗体和基因重组人IL-2共同诱导人外周血单个核细胞，制备抗CD3单克隆抗体活化的杀伤细胞（CD3AK）。
Human CD3AK cells were prepared from peripheral blood mononuclear cells by culturing them in recombinant IL-2 and anti CD3McAb.
应用抗CD3单克隆抗体和基因重组人IL-2共同诱导人外周血单个核细胞，制备抗CD3单克隆抗体活化的杀伤细胞（CD3AK）。
Human CD3AK cells were prepared from peripheral blood mononuclear cells by culturing them in recombinant IL-2 and anti CD3McAb.
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