方法用PCR方法扩增il_3信号肽序列和人内皮抑素基因，然后采用重叠延伸pcr拼接的方法将两者拼接和扩增。
METHODS IL_3 signal sequence and human endostatin gene were amplified with PCR and spliced with overlap extension PCR.
目的观察人脐静脉内皮细胞株(ECV304细胞)在内皮抑素(es)作用前后基因表达谱的变化，并探讨其作用的分子机制。
Objective to observe the altered gene expression of ECV304 cell after treated with endostatin (es) using cDNA microarray, and to investigate its molecular mechanism.
目的观察人脐静脉内皮细胞株(ECV304细胞)在内皮抑素(es)作用前后基因表达谱的变化，并探讨其作用的分子机制。
Objective to observe the altered gene expression of ECV304 cell after treated with endostatin (es) using cDNA microarray, and to investigate its molecular mechanism.
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