串联亲和纯化是目前应用最广泛、最具优势的方法之一。
One of the most advantageous and widely used method is the tandem affinity purification.
亲和纯化后抗体的纯度达99%以上。
After purification, the purity of the moAbs could reach more than 99%.
目的用亲和纯化法纯化嗜水气单胞菌气溶素，从单因子水平分析嗜水气单胞菌的溶血特性。
Methods:The proteins which interacted with gankyrin were screened and analyzed by affinity purification technology and mass spectrometry.
结论GST-3C蛋白酶不仅可以应用于融合蛋白的酶切，而且为引入3C蛋白酶识别位点的串联亲和纯化（TAP）系统提供又一种酶切工具。
Conclusion The recombinant GST-3C protease can be not only applied to the cleavage of fusion proteins, but also be used as a tool-enzyme in TAP system containing 3C recognition sequence.
多克隆抗体由合成化肽段免疫动物，该合成肽段与YAP人源蛋白序列一致，抗体由蛋白a和肽段亲和层析技术纯化。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the human sequence of YAP. Antibodies are purified by protein a and peptide affinity chromatography.
多克隆抗体通过用DYKDDDDK合成肽免疫动物制备。抗体用蛋白a和多肽亲和色谱法纯化。
Polyclonal antibodies are produced by immunizing animals with a synthetic DYKDDDDK peptide. Antibodies are purified by protein a and peptide affinity chromatography.
抗体由蛋白A和肽段亲和层析纯化得到。
Antibodies are purified by protein A and peptide affinity chramotography.
表达产物用亲和层析一步法进行纯化，然后用凝胶阻滞试验观察重组蛋白结合质粒的能力。
The expressed protein was purified by one-step affinity chromatography, and its ability of binding plasmid was investigated via gel retardation experiments.
抗体采用蛋白a和肽亲和层析法纯化。
Antibodies are purified by protein a and peptide affinity chromatography.
该多克隆抗体由合成的人类CDK5氨基酸序列对应肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human CDK5. Antibodies are purified by protein A and peptide affinity chromatography.
抗体由蛋白a和肽段亲和层析纯化得到。
Antibodies are purified by protein a and peptide affinity chromatography.
抗体由蛋白a和肽段亲和层析技术纯化。
Antibodies are purified by protein a and peptide affinity chromatography.
结论用尼龙滤膜zbm改进的膜亲和层析法，可用于纯化腹水中的单克隆抗体，且简便、省时、有效。
Conclusion The modified MAC with ZBM is a much easier, time-saving and effective method for affinity purification of antibodies in ascites.
在非变性条件下，用TALON金属亲和层析树脂纯化含组氨酸标记的重组DHFR。
The recombinant DHFR protein containing his-tag was purified by using TALON metal affinity resin under nondenaturing conditions.
该多克隆抗体用与人类PKM2蛋白氨基酸序列对应的人工合成肽段免疫动物制成。该抗体使用蛋白A和肽亲和层析纯化而得。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PKM2. Antibodies are purified by protein A and peptide affinity chromatography.
该多克隆抗体用与人类LDHA氨基酸序列对应的人工合成肽段免疫动物制成。该抗体使用蛋白亲和层析纯化而得。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human LDHA. Antibodies are purified by peptide affinity chromatography.
制备了以壳聚糖为载体的金属螯合亲和色谱分离纯化还原型谷胱甘肽(GSH)。
Immobilized metal ion affinity chromatography sorbents were prepared for purification of reduced glutathione (GSH) using chitosan as substrate.
多克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与包围于人源APC蛋白序列一致，抗体由蛋白a和肽段亲和层析技术纯化。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human APC. Antibodies are purified by protein a and peptide affinity chromatography.
该多克隆抗体用与人类己糖激酶i氨基酸序列对应的人工合成肽段免疫动物制成。该抗体使用蛋白a和肽亲和层析纯化制得。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human hexokinase I. antibodies are purified by peptide affinity chromatography.
方法采用优球蛋白沉淀、离子交换色谱、(NH4)2so4沉淀和亲和色谱法进行纯化。
Methods a combination of euglobulin precipitation, ion-exchange chromatography, (NH4) 2so4 precipitation and affinity chromatography was used in the process of purification.
IPTG诱导该基因的表达，亲和层析和原位裂解法纯化表达产物。
The modified gene was expressed by IPTG induction and purified by affinity chromatography and cleavage in place.
IPTG诱导表达融合蛋白，包涵体蛋白经复性后亲和层析法纯化，ELISA方法测定蛋白活性。
Fusion protein expression was induced by IPTG. After renaturation, the protein was purified by affinity chromatography and the bioactivity was examined by ELISA.
建立杂交瘤单抗亲和层析纯化抗原、抗原体外致敏淋巴细胞和RT-PCR克隆人抗体基因及噬菌体呈现技术构建人源抗体库的策略。
Strategy was established for construction of repertoire antibody library with affinity chromatography purifying antigen, antigen immunizing human lymphocytes, RT-PCR and phage display technology.
亲和层析是基于生物分子间特异性的亲和作用而建立的分离纯化技术。
Affinicty chromatography is a separating and purifying technique based on the specific affinity between biomolecules.
用谷胱甘肽琼脂糖凝胶4b亲和介质从菌体裂解液中纯化了GST -MBD4融合蛋白。
The GST-MBD4 fusion protein was purified from cell lysates using glutathione Sepharose 4b affinity chromatography.
包涵体蛋白经含8m尿素的结合缓冲液溶解后，用金属亲和层析法进行纯化，获得了高纯度的目的蛋白。
After dissolution of inclusion bodies in binding buffer containing 8m Urea, the fusion protein was purified with metal affinity chromatography column, high purity protein was achieved.
亲和膜分离技术是一项新兴的技术，兼有膜分离和亲和色谱的优点，可以有效地进行生物产品的分离和纯化。
Affinity membrane separation, a new technology, has advantages of both membrane separation and affinity chromatography, and is good for the separation and purification of biological products.
用高速离心、分段盐析、亲和层析、超滤和阴离子交换层析法，从牛胎盘中分离纯化出一种肝细胞生长因子。
A kind of hepatocyte growth factor was purified from cow placenta through high speed centrifugation, salting out, affinity chromatography, ultrafiltration and anion exchange chromatography.
该抗体通过使用合成肽段免疫动物而获得，并经蛋白a和肽亲和层析纯化。
Antibodies are produced by immunizing animals with synthetic peptides, and are purified by combinations of Protein a and peptide affinity chromatography.
该抗体通过使用合成肽段免疫动物而获得，并经蛋白a和肽亲和层析纯化。
Antibodies are produced by immunizing animals with synthetic peptides, and are purified by combinations of Protein a and peptide affinity chromatography.
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