目的用生物素-链霉亲合素放大elisa系统建立一种敏感、特异、经济简便的抗环瓜氨酸肽(CCP)抗体的测定法。
Objective To develop a highly sensitive, specific and simple enzyme linked immunosorbent assay (ELISA) for determination of anti-cyclic citrullinated peptide (CCP) antibody.
方法：利用亲合层析及凝胶过滤层析从小鼠黑色素瘤中提取小鼠黑色素瘤凝集素（MMA）。
Methods: MMA was prepared with asialo-fetuin affinity and gel-filtration chromatography form murine melanoma.
采用定量酶细胞化学、凝集素亲合细胞化学、透射电镜、扫描电镜及吞噬实验观察P M的结构和功能变化。
The structure and function of PM were observed with quantitative cytochemistry, lectinaffinitive cytochemistry, transmission and scanning electron microscope and phagocytic test.
采用定量酶细胞化学、凝集素亲合细胞化学、透射电镜、扫描电镜及吞噬实验观察P M的结构和功能变化。
The structure and function of PM were observed with quantitative cytochemistry, lectinaffinitive cytochemistry, transmission and scanning electron microscope and phagocytic test.
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