以二维电泳为基础的蛋白质组学是发现蛋白磷酸化状态改变的有效途径。
And proteomics technology based on two-dimensional electrophoresis is an effective approach to discover the changes in the states of protein phosphorylation.
用十二烷基硫酸钠-凝胶电泳(SDS -PAGE)和二维凝胶电泳鉴定蛋白质纯度。
The obtained proteins were checked for the purity through SDS-polyacrylamide gel electrophoresis (SDS-PAGE).
目的：利用二维凝胶电泳法分离人肾组织磷酸化蛋白质组。
Objective: To separate human kidney phosphoproteome by two-dimensional gel electrophoresis.
用二维电泳法比较分析两种按蚊卵的蛋白质多肽图谱。
The polypeptide patterns of the eggs of Anopheles sinensis and An. anthropophagus were compared with two-dimensional electrophoresis in polyacrylamide gel.
常见的用于宿主细胞蛋白的分析的技术包括酶联免疫吸附法(酶联免疫吸附试验)，一维或二维凝胶电泳，免疫印迹和质谱分析。
Common technologies used for HCP analysis include ELISA (Enzyme-linked Immunosorbent Assay), one-dimensional or two-dimensional gel electrophoresis, Western Blot and Mass Spectrometry.
蛋白质组学部分承用了创立于二十多年前的二维电泳技术。
Proteomics has its origins in two dimensional gel electrophoresis (2 DE), a technique developed more than twenty years ago.
建立并优化白念珠菌总蛋白质组分析的二维电泳条件。
To develop and optimize a two-dimensional(2-DE) gel electrophoresis condition for Candida albicans proteome analysis.
方法利用二维凝胶电泳获得新生隐球菌孵育后血管内皮细胞与正常细胞差异表达蛋白质点，并对部分差异蛋白点进行质谱鉴定分析。
Methods The differentially expressed proteins between HUVEC incubated with Cryptococcus and normal HUVEC were obtained by 2-D electrophoresis and identified by MALDI-TOF MS technology.
最后我们将优化的蛋白质组学样品制备方法应用于鸡的脾脏和胸腺，也获得了高质量的二维电泳图谱。
In addition, this optimized protocol was also successfully applied in 2-DE analysis of spleen and thymus in chicken.
最后我们将优化的蛋白质组学样品制备方法应用于鸡的脾脏和胸腺，也获得了高质量的二维电泳图谱。
In addition, this optimized protocol was also successfully applied in 2-DE analysis of spleen and thymus in chicken.
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