目的以原代培养小鼠骨髓基质细胞为饲养层，探讨精原干细胞能否在该饲养层上生长及生长的影响因素。
To study whether spermatogonial stem cells can grow on the feeder layers of bone marrow stromal cells, and their effecting factors in vitro.
目的： 分离培养睾丸支持细胞、骨髓基质细胞、胚胎成纤维细胞和胰腺成纤维细胞，优化培养条件，建立饲养细胞系，为干细胞体外共培养体系的建立提空充足的饲养细胞。
Objective: Sepretae Sertoli cell, BMSC, MEF, Pancreatic fibroblast and establish cell lines, constructed different feeder layers for a source as a coculture system for stem cells.
目的： 分离培养睾丸支持细胞、骨髓基质细胞、胚胎成纤维细胞和胰腺成纤维细胞，优化培养条件，建立饲养细胞系，为干细胞体外共培养体系的建立提空充足的饲养细胞。
Objective: Sepretae Sertoli cell, BMSC, MEF, Pancreatic fibroblast and establish cell lines, constructed different feeder layers for a source as a coculture system for stem cells.
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