前3次免疫时采用的是弗氏完全佐剂乳化,后3次免疫采用弗氏不完全佐剂乳化。
The antigen had been emulsified with Freund' s complete adjuvant in the earlier 3 times and with Freund' s incomplete adjuvant in the latter 3 times.
PAGE电泳后,将表达的CP蛋白切胶回收,研磨成粉后加入等体积的福氏不完全佐剂,乳化制备成抗原。
After SDS-PAGE, the recovery of CP added the same volume of incomplete Freund Adjuvant emulsified completely, injected rabbits by subcutaneous injection.
PAGE电泳后,将表达的CP蛋白切胶回收,研磨成粉后加入等体积的福氏不完全佐剂,乳化制备成抗原。
After SDS-PAGE, the recovery of CP added the same volume of incomplete Freund Adjuvant emulsified completely, injected rabbits by subcutaneous injection.
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