在锤头型核酶下游增加一段核酶作用的靶序列，使之成为自切割的核酶。
A self cleaving ribozyme was comprised of a hammerhead ribozyme and its target sequence located the downstream of the hammerhead ribozyme.
在FMDV的核苷酸序列相对保守区设计上下游引物FM1/FM4，扩增病毒的基因片段。
Then capture FMDV from clinical material with sterilized Eppendrof tubes of coating type-specific IgG, Amplify the conserved viral sequences by common primers (FM1/FM4).
应用谱分析对长江下游和西江下游感潮河段水文序列进行了周期识别，获得了可信的隐含周期。
The cryptic period of the hydrologic time series of tidal reaches of the Yangtze River and Xijiang River are analyzed with the applying of spectral analysis and the result is reasonable.
重组体用克隆位点上游和下游的T7和T3启动子序列为测序引物，用自动测序仪测序鉴定克隆的正确性。
The recombinant was sequenced by automatic sequencer with promoters T7 in upstream and T3 in downstream as sequencing primers.
因此选择对基因的表达具有重要功能的外显子1和其上游的调控区及下游外显子内含子交界区序列通过直接测序法进行SNP筛查，并进一步分析多态位点与病窦的关联性。
Therefore the other aim of our study is to discover SNPs of HCN4 exon 1, including the regulatory sequence and exon-intron boundary of it, which plays an importable role of channel function.
方法1。根据HSP70全长基因序列设计针对其编码区的引物，在下游引物的宁端加人MG7模拟表位的核昔酸序列。
Methods (1) The coding sequence of MG7 mimotope (GC23) was included in the reverse primer, and by PCR incorporated with HSP70 gene at the 3 terminus.
方法1。根据HSP70全长基因序列设计针对其编码区的引物，在下游引物的宁端加人MG7模拟表位的核昔酸序列。
Methods (1) The coding sequence of MG7 mimotope (GC23) was included in the reverse primer, and by PCR incorporated with HSP70 gene at the 3 terminus.
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