目的研究人胃黏膜上皮细胞系无嘌呤无嘧啶核酸内切酶(APE)的表达状况。
Objective To study the expression of APE/Ref-1 in gastric mucosal epithelial cell lines.
方法用1%多聚甲醛固定嗜酸粒细胞和支气管上皮细胞系BEAS-2B细胞;
Methods Eosinophils and BEAS-2B cells were fixed with 1 % paraformaldehyde for 1h separately.
人晶状体上皮细胞系内肌醇1,4,5-三磷酸受体和兰尼碱受体的表达和作用。
Expression and role of inositol 1,4,5-trisphosphate receptor and ryanodine receptor in a human lens epithelial cell line.
经反复传代,初步建立了人胚胎角膜上皮细胞系,并对其生物学特性进行了研究。
After human embryo corneal epithelial cell line was established through many cell generations, its biological character was researched.
结论:经鉴定,ADPKD囊肿衬里上皮细胞系已建立,可用于ADPKD细胞及分子生物学的研究。
CONCLUSION an ADPKD cyst lining epithelial cell line has been established and identified, which can be used in the research of ADPKD cellular and molecular mechanism.
首先,应用过氧化氢处理肠上皮细胞系SW- 480细胞建立活性氧诱导肠上皮细胞凋亡的体外模型。
Firstly, hydrogen peroxide-induced apoptosis of SW-480 cells, a kind of intestinal epithelial cells line, was established.
体外试验中,正常细胞形态相似,腺泡结构排列整齐,然而那些高侵袭的上皮细胞系的结构紊乱并且失去极性。
Normal cells form similar, well-organized acinar structures in vitro, whereas in highly metastatic epithelial cell lines these structures are disorganized and lack polarity.
方法:采用逆转录聚合酶链反应(RT - PCR)、流式细胞仪和免疫荧光细胞染色分析技术分析肺上皮细胞系a549细胞PAR s的表达情况。
METHODS: We used RT-PCR, flow cytometry and immunofluorescence cell staining techniques to observe the expression of PARs on A549 cells.
因此,人胚胎角膜上皮细胞适合于建立相对稳定的细胞系。
Thus, the human embryo corneal epithelial cells was fit for establishing a relative stable cell line.
方法6个喉癌细胞系和4例喉癌旁正常组织上皮细胞培养。
METHODS Epithelial cell culture from 6 laryngeal carcinomas and 4 normal laryngeal tissues were conducted.
方法6个喉癌细胞系和4例喉癌旁正常组织上皮细胞培养。
METHODS Epithelial cell culture from 6 laryngeal carcinomas and 4 normal laryngeal tissues were conducted.
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