目的了解角膜上皮干细胞的生长特性。
Objective To explore the growth features of corneal epithelial stem cells in vitro.
方法利用无血清悬浮培养、单细胞克隆技术分离神经上皮干细胞;
Methods Serum free cells suspension culture and single cell cloning technique were used to isolate neuroepithelial stem cells.
现综述近年来有关上皮干细胞和肿瘤发生的关系及其调控机制方面的研究进展。
This article reviews the relationship and regulatory mechanism between normal stem cells and epithelial tumor stem cells.
目的从胚胎大鼠脊髓神经管中分离神经上皮干细胞并诱导其向多巴胺能神经元方向分化。
Objective to isolate neuroepithelial stem cells from the spinal cord neural tube of the embryonic rat and induce them to differentiate into dopaminergic neurons.
我们提出假说,即鼠类磨牙具有维持上皮干细胞龛的内在潜能,并通过体外实验来证明此假说。
We hypothesized that the mouse molar has intrinsic potential to maintain the epithelial stem cell niche and assessed this potential by growth in vitro.
结论胎儿角膜上皮干细胞呈现由全层向基底层,再向缘部逐渐迁移的分布规律,且超微结构较幼稚。
Conclusion the migration of corneal epithelial stem cells in the human fetuses was from the whole layers to basal layer and confined to limbus region finally, and their ultrastructure was immature.
所以,人们认为肿瘤多起源于正常干细胞。然而,目前研究中关于上皮肿瘤干细胞及其与正常上皮干细胞的关系了解甚少。
So it is generally assumed that tumor cells are derived from normal stem cells, more work is needed to identify and characterize epithelial tumor stem cells.
这些干细胞是毛发生长的根源,而且可以在创伤后修复滤泡和周围的上皮组织。
These stem cells are responsible for driving this cycle of growth and can repopulate follicles and surrounding epidermis damaged by wounding.
角膜缘干细胞是角膜上皮更新的源泉,维持角膜完整性。
Corneal limbus stem cells are the source of corneal epithelial renewal and they help maintain the integrity of cornea.
具体来说,鲜为人知的是基底细胞的增殖和分化的假气道上皮细胞,多能干细胞群。
Specifically, little is known about the proliferation and differentiation of basal cells, a multipotent stem cell population of the pseudostratified airway epithelium.
研究结果证实脊索瘤中既有上皮组织分化的特点又有间叶组织的表现,还存在着具有多潜能分化的恶性干细胞群体。
Some cells were multipotential neoplasms cells in which the stem cells were capable of differentiation along both epithelial and mesenchymal pathways.
结论角膜及角膜缘表层上皮较为成熟分化,而角膜缘基底细胞具有未分化细胞的特征,很可能是干细胞的部位。
ConclusionThe superficial cells of limbus and cornea are of mature status, while limbal basal cells show some undifferentiated feature, which indicates the location of stem cells.
结论:胚胎神经上皮细胞多数为神经干细胞,黑质内移植后可以存活并分化为多巴胺能神经元。
Conclusion: Present findings demonstrate that embryonic neural stem cells dissociated from neural tube can survive and differentiate into dopamine neurons.
通过我们的研究,实现了由小鼠胚胎干细胞向纤毛上皮细胞的分化,并且分化细胞具有正常纤毛细胞的特性。
In the present study, we demonstrated that the mouse embryonic stem cells were differentiated into ciliated epithelial cells, with characteristics of normal ciliated cells.
新鲜板层角膜含健康的角膜缘干细胞和角膜上皮细胞,可迅速修复损伤的角膜表面。
Fresh lamellar cornea contains health corneal limbus stem cells and epithelial cells, it can repair the trauma corneal surface soon.
采用这些标记复合体,可将角膜缘干细胞群与其他上皮细胞区分。
With these markers complex, it is easy to distinguish limbal stem cells from other epithelial cells in cornea.
目的:用体外培养的角膜缘上皮细胞联合人羊膜的方法,行自体移植治疗角膜缘干细胞完全缺乏的兔眼。
Objective: to study in vitro cultivated limbal epithelial cells autograft with amniotic membrane grafts for total limbal stem cell deficiency of rabbit eyes.
利用干细胞,使之成为视网膜色素上皮细胞他们的供应几乎是无限的。
By using stem cells and turning them into retinal pigment epithelium cells, the supply is virtually unlimited.
在小鼠呼吸道表面(支气管上皮)发现的干细胞在病毒感染后快速增殖并迁移到损伤部位。
Stem cells found along the surfaces of the airways (in the bronchiolar epithelium) proliferate rapidly in mice after viral infection and migrate to sites of damage.
目的采用无饲养层细胞培养体系构建家兔角膜上皮组织,自体移植后观察其对角膜缘干细胞缺失的治疗效果。
Objective to reconstruct rabbit corneal epithelium in vitro with a free-feeding layer culture system and autograft to treat limbal stem cells deficiency.
结论采用无饲养层细胞培养体系构建自体角膜上皮组织,移植后可以成功修复角膜缘干细胞缺失的角膜上皮。
Conclusion the reconstructed rabbit corneal epithelium hi vitro with a free-feeding layer culture system could be autografted to repair total corneal limbal stem cells deficiency.
目的:观察上皮型钙粘蛋白在小鼠胚胎干细胞体外分化过程中的动态表达,及其与细胞间黏附状态的关系。
OBJECTIVE: To observe the dynamic expression of E-cadherin in embryonic stem cell differentiation and the effect on cell adhesion.
目的:探讨羊膜上皮细胞是否能在体外促进胚胎脑神经干细胞的存活及分化。
Objective Whether amniotic epithelial cells could promote the survival and differentiation of neural stem cells was explored in vitro.
结论大鼠骨髓间充质干细胞对生精上皮放射性损伤的修复可起重要作用。
Conclusion MSCs can play an important role in repair of radiation injury of spermatogenic epithelium.
目的探讨人12周龄胚胎脑脉络丛上皮细胞是否具有神经干细胞的生物学特性。
Objective In order to investigate whether the 12-week-old embryo brain choroid plexus epithelial cells have neural stem cell biological properties.
角膜缘部上皮基底层的干细胞具有细胞更新和组织再生的功能,而眼部表面疾病则是干细胞缺乏的结果。
The stem cells of the basal layer of the limbal epithelium have function of cell renewal and tissue regeneration, and ocular surface disorders result from lack of the stem cells.
角膜上皮的增生与更新依赖于角膜缘干细胞,角膜缘干细胞缺乏可导致视力障碍。
The proliferation and refreshment of corneal epithelium depends on corneal limbal stem cells. Corneal limbal stem cell deficiency can lead to visual disorder.
角膜缘干细胞的发现是十几年来眼科最重要的进展之一,现已公认,角膜缘干细胞是角膜上皮细胞再生的最终源泉。
The discovery of limbal stem cell is one of the most important advances in ophthalmologic field. It is now accepted that limbal stem cell is the ultimate source of corneal epithelium regeneration.
我们以前的研究表明在兔晶状体中央区上皮细胞中可能存在晶状体干细胞,故本研究目的之一是进一步证实我们之前的结论。
Our previous studies suggest the existence of lens stems cell in the central epithelium of rabbit lens. One of the purposes of the present study is to confirm our previous conclusion.
使用本发明的载体将基因导入到呼吸道上皮组织干细胞可基因治疗囊性纤维症等遗传性呼吸系统疾病。
This gene transfer into airway epithelial stem cells with the use of the vector as described above is useful in gene therapy for a hereditary respiratory disease such as cystic fibrosis.
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