目的探讨血清对白念珠菌菌相转换特异基因HYR1上游启动子活性的影响。
Objective To study the effect of serum on promoter activity of Candida albicans yeast-hyphal regulational gene(HYR1).
随后人GRP94启动子克隆了荧光素酶基因上游区且缺氧环境中活性增强。
The human GRP94 promoter was then cloned upstream of the luciferase gene and showed enhanced activity in hypoxic conditions.
重组体用克隆位点上游和下游的T7和T3启动子序列为测序引物,用自动测序仪测序鉴定克隆的正确性。
The recombinant was sequenced by automatic sequencer with promoters T7 in upstream and T3 in downstream as sequencing primers.
重组体用克隆位点上游和下游的T7和T3启动子序列为测序引物,用自动测序仪测序鉴定克隆的正确性。
The recombinant was sequenced by automatic sequencer with promoters T7 in upstream and T3 in downstream as sequencing primers.
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