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Yuto Nagamoto latched onto Keisuke Honda's through ball and squared for Ryoichi Maeda to level the tie up at 1-1.
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When they did break up the Netherlands' passing, Grenoble midfielder Daisuke Matsui looked bright for Japan - instigating an incisive move that eventually resulted in Yuto Nagatomo hitting a stinging shot just wide.
BBC: Netherlands 1-0 Japan
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"Our experiments have so far shown that MC26 causes few side-effects, definitely much less than amantadine, " said Yuto Kamei, an assistant professor of marine biotechnology and one of those who led the study.
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Seconds later, referee Hector Baldassi waved away penalty claims as Yuto Nagatomo went down under a challenge from Manchester City midfielder Nigel de Jong and the Dutch clung on to achieve maximum points from their opening two fixtures.
BBC: Netherlands 1-0 Japan