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James Silvey, from the Trossachs Water Vole Project, said the voles now had an "established population" in the forest.
BBC: Water voles back in the Trossachs
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Water vole numbers have crashed due to predation and loss of habitat.
BBC: Lincolnshire project restores water vole streams
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Chalk streams in the Lincolnshire Wolds are shallow, clear and oxygen rich, supporting a wide variety of plants and wildlife, including the water vole.
BBC: Lincolnshire project restores water vole streams
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Steve Lowe, from the Northumberland Wildlife Trust, said a similar project had been carried out in the Cairngorms, which saw the water vole making an "impressive comeback".
BBC: Water vole
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Other organisations involved in the water vole reintroduction include the Royal Zoological Society of Scotland, Scottish Natural Heritage, Loch Lomond and the Trossachs National Park and water vole expert Derek Gow.
BBC: Water voles back in the Trossachs
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"We hope that this project will not only see the waterwheel turning again for the first time in decades, but will also see a return of water vole and other species to the mill pond and leetes, " he said.
BBC: Loggerheads Country Park: Mill waterwheel to turn again