In mice, Daley and colleagues have shown that stem cells derived from the nuclear transfer of cells to make embryos -- the technique described in Mitalipov's paper -- were indeed closer to natural embryo stem cells than induced pluripotent stem cells.
Only last year, she said, Egli and Paull and their colleagues demonstrated that the nuclear transfer of eggs cells could generate patient-specific stem cell lines for potential cell replacement therapy to treat diseases like diabetes.
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The liver is Geron's first target in its plan to use cloning technologies, called nuclear transfer, to produce transplantable cells and organs from the patient's own body.
Here again, losses occur, and only 10% of the original egg cells used in nuclear transfer make it this far.
Unsupported claims that the 35S promoter is unstable, prone to transfer and insertion into the DNA of other cells, thereby causing cancer in humans, have been extensively rebutted by the scientific community and are without merit.
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The problem is that making them using somatic-cell nuclear transfer involves breaking up viable embryos, since the cells in question are found inside early-stage embryos, called blastocysts.
Instead of generating embryonic stem cell lines via nuclear transfer, Hwang's group produced the stem cells from days-old embryos, a technique that had already been established by James Thomson at University of Wisconsin in 1998.
Geron , the stem-cell company he founded before moving to ACT, has often talked of nuclear transfer as a way of figuring out how to make stem cells.
Geron (nadsaq: GERN - news - people ), the stem-cell company he founded before moving to ACT, has often talked of nuclear transfer as a way of figuring out how to make stem cells.
Indeed, the same objection also applies to stem cells that are extracted from embryos produced without nuclear transfer. (Usually these are surplus to requirements for in vitro fertilisation.) Both problems would go away if cells that behave like embryonic stem cells could be made without destroying embryos.
With this project she will develop a new label-free biomolecular detection method, based on a technique called Plasmon Resonance Energy Transfer (PRET), to monitor biochemical pathways involved in the differentiation of stem cells.
The gold standard embryonic stem cells still came from embryos themselves, including ones that were made through nuclear transfer.
Instead, they want to use somatic cell nuclear transfer, the same process used to clone Dolly the sheep in 1997, to create embryonic stem cells.
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