TransEuropa had run passenger and freight services between Ramsgate and Ostend for the last 15 years.
The development means that there is no passenger service out of the Port of Ramsgate.
The temporary ban was enforced last month after two incidents at the Port of Ramsgate.
BBC: Thanet council legal fight over Ramsgate live exports ban
Last month, Thanet council was ordered to resume live exports from Ramsgate by the High Court.
Activists are holding a 24-hour vigil at Ramsgate port to celebrate the ban on exports.
The National Farmers' Union has called on the Port of Ramsgate to resume live animal exports.
Belgian firm TransEuropa suspended all sailings between Ramsgate and Ostend this week amid fears over its future.
But it said that events at Ramsgate in the latter half of 2012 went beyond peaceful protest.
Two weeks ago, the Port of Ramsgate stopped exports after 45 sheep died in two separate incidents.
Plans to extend Kent's high-speed rail line from Ashford to Ramsgate have been discussed with a government minister.
Last year Thanet council banned live exports from Ramsgate, but the ban was later overturned by the High Court.
BBC: Second Dover berthing trial of animal export ship MV Joline
The MV Joline, which has been operating from Ramsgate in Kent, is hoping to berth at the Eastern Docks.
BBC: Second Dover berthing trial of animal export ship MV Joline
The charity said the birds were found covered in thick black fuel oil off Ramsgate and Rye last week.
Thanet council banned live exports from Ramsgate last year but the ban was later overturned by the High Court.
BBC: Dover trial of animal export ship MV Joline inconclusive
Three men and a firm are to face charges following the deaths of 47 sheep at the Port of Ramsgate.
The stricken birds were all found in the Ramsgate harbour area except for one red-throated diver, which was in Rye harbour.
If they win they will become the first Kent League side to reach the first round since Ramsgate Athletic in 1956.
Circumstances which led to the slaughter of 44 sheep at the Port of Ramsgate were "absolutely unacceptable", the government has said.
Dover Harbour Board halted the trade in April 2011, prompting the move to Ramsgate which is owned by Thanet District Council.
When the ship returned to Ramsgate our passengers were whisked away, we fuelled, stored, tidied up and it was time to go again.
Thanet Borough Council, which covers Margate, Ramsgate and Broadstairs, did not have exact figures, but said they were 90% of those it handled.
More than 80 residents want developers to be thrown off Ramsgate's former Pleasurama site amid complaints it has become derelict, a councillor has said.
BBC: Ramsgate residents call to 'tear up' seaside flats plan
She said she was "fed up" with a trade that had led to the slaughter of dozens of sheep on the portside at Ramsgate.
Sir Roger Gale told the Commons the sheep on the vessel had a further journey after their return to Ramsgate and suffered "absolute misery".
The RSPCA has failed in a renewed attempt to challenge the legality of the export of live sheep through the port of Ramsgate in Kent.
On Monday, the Farming Minister David Heath announced that every future consignment of live animals that passed through the Port of Ramsgate would be inspected.
Live animal exports need to resume from the Port of Ramsgate following their suspension after the death of 45 sheep, the National Farmers' Union says.
Thanet District Council suspended live exports through the Port of Ramsgate on 20 September and live animals were sent abroad from the Port of Ipswich.
Dover Harbour Board (DHB) said Barco de Vapor, which currently operates from Ramsgate in Kent, had applied to berth the MV Joline from the Eastern Docks.
The Joline set off from Ramsgate on Wednesday with two lorry loads of sheep, but the captain aborted the crossing because of strong winds and rain.
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