Researchers at the Cornell University Nanobiotechnology Center are combining RT-PCR with a nanofluidics-based system for separation and identification of genetic material from different organisms.
Real-Time Polymerase Chain Reaction (RT-PCR) uses nanoscaled fluorescent probes that allow the tracking of the progress of the amplification from the start.
The authors expressed surprise that their PCR approach produced gag sequences which did not contain the 24-nt deletion characteristically present in the XMRV sequences cloned to date, as they expected their primers to be specific to VP62 gag.