Pop the PCR output in the mail and Cofactor will send back the data.
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However, PCR is relatively complex to carry out, expensive, and does not produce quick results.
Therefore the amount of mtDNA actually recoverable by PCR in this experiment may well have been minimal.
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In a typical PCR machine a large aluminum block is used to heat and cool the DNA samples.
Ms. Dewey-Hagborg amplifies small DNA strands using a technique called PCR polymerase chain reaction.
These kits are intended to let unskilled people hone in on a genetic target and amplify it using a PCR machine.
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The virus' genetic code was uncovered with one of the most common tests in biology, the polymerase chain reaction, or PCR.
Real-Time Polymerase Chain Reaction (RT-PCR) uses nanoscaled fluorescent probes that allow the tracking of the progress of the amplification from the start.
The occurrence of Toxoplasma gondii and Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from eastern Poland with the use of PCR.
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This of course means that any conclusion made by PCR alone regarding the association of XMRV and mouse DNA is profoundly unsafe.
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The authors theorise that this may be due to the inability of their PCR assay to detect XMRV in low copy numbers.
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Researchers at the Cornell University Nanobiotechnology Center are combining RT-PCR with a nanofluidics-based system for separation and identification of genetic material from different organisms.
And while Life is completely defocusing all volume related business such as PCR QPCR and cell sciences, they are also focusing on making less money.
Dr Lee accepts that the new test is not quite as sensitive as one of the alternatives, the PCR test, which detects DNA samples from chlamydia.
These chemicals may speed one of the most fundamental reactions in genetic research, called polymerase chain reaction (PCR), by allowing scientists to perform it at higher temperatures.
For years, he had been making the case that polymerase chain reaction (PCR) would be the best way to test for the existence of Earth-like DNA on Mars.
In 1993, Kary Mullis won a Nobel Prize for discovering PCR, and several widespread efforts, including the Human Genome Project, have used the science to compile their databases.
Real-time PCR quantitation of clostridia in feces of autistic children.
It is difficult to fathom therefore why the authors would conclude that these expected PCR findings were evidence of mouse contamination when a far more parsimonious explanation was available.
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Next we turn to the section of the study where the authors test the ability of their PCR assays (IAP and mtDNA) to detect mouse DNA in mouse cell lines.
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The detection rate by PCR is in line with that reported by Danielson et al but some 400% lower than that achieved by IHC using XMRV specific probes (2, 3).
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One example is the polymerase chain reaction (PCR), a standard technique which employs enzymes to churn out millions of copies of a DNA sequence and involves cycles of gentle heating and cooling.
Similarly the claim that IAP is superior to , or even as reliable , as mtDNA PCR in vivo is not supported by the data cited as alternative explanations for the findings are readily available.
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Current detection tools using nanotechnology allow high-speed molecular detection of pathogens through analysis of their DNA in a matter of a few minutes where classical detection tools using regular PCR (polymerase chain reaction) would take over six hours.
As PCR is by some considerable margin the least sensitive method for detecting XMRV in prostate cancer (2, 3) and chronic fatigue syndrome (4) no association between the presence of mouse DNA and XMRV can be safely made.
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The authors expressed surprise that their PCR approach produced gag sequences which did not contain the 24-nt deletion characteristically present in the XMRV sequences cloned to date, as they expected their primers to be specific to VP62 gag.
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The polymerase chain reaction (PCR) is a scientific technique in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.
Life Technologies, formed by the merger last year of life science tools companies Applied Biosystems and Invitrogen, has an estimated 65% of the market for PCR machines used for research and has licensed its technology to 12 other companies making the machines.
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