The defeat leaves Vale seven points shy of a play-off spot with eight games left to play.
Joe Ledley gave the Bluebirds the play-off spot with two games to spare.
The Tigers stole the final Premiership play-off spot from Quins when winger Tom Varndell scored in the 77th minute.
Scunthorpe had only secured a play-off spot courtesy of an 88th-minute Cliff Byrne goal on the final day of the season.
Second-placed France , already assured of a play-off spot, avenged their defeat in Austria with a comfortable 3-1 victory in their final qualifier.
They must beat Hungary and Malta in their last two matches, both at home, to salvage any realistic hope of a play-off spot.
The Shrimpers secured their fourth win in five with a 2-0 defeat of Scunthorpe on Tuesday and are now 10 points from a play-off spot.
However, it's highly unlikely that the Slovenians will slip up against the minnows and Northern Ireland now look set to miss out on a play-off spot.
Forest are now on 40 points, two behind sixth-place Watford, but have played up to three games fewer than some of their rivals for a play-off spot.
Of course, for the Scots to clinch a coveted play-off spot, it's highly likely they will first need to overcome the odds and beat the Dutch at Hampden.
The visitors are still pinning their hopes on a series of unlikely results to book a play-off spot and the Brewers are still favourites to go up as champions.
Magilton took Ipswich to 14th in the Championship table in his first season and eighth last term but was sacked in April after failing to secure a play-off spot.
The home team will face Costa Rica who had to settle for the play-off spot after a 2-2 draw with the already-qualified United States having taken an early two-goal lead.
Champions Leicester had began the day in sixth, needing a bonus-point victory over the Quins, as well as Sale losing to London Irish to qualify for the final play-off spot.
The club were docked 10 points by the Football League which effectively ended their hopes of a play-off spot in League Two, and a new buyer was sought by the administrators.
Olofinjana finally broke the deadlock and the teams played eight minutes of added time but Wolves could not find the two more goals they needed to overhaul Watford and snatch the final play-off spot.
But the Seasiders lost just once in their remaining nine games as they came from nowhere to sneak into the last play-off spot - at Swansea's expense - on the final day of the season.
Although the Rodney Parade-based side sit third from bottom in the Magners League, just one point from Friday night's home match against Connacht will ensure a play-off spot to return to the top-tier Heineken Cup next season.
Logic dictates that the nature of Denmark's qualification for the Euro 2000 finals - coming back from a disastrous start, indeed a disastrous middle section too, to sneak the play-off spot in their group - owes nothing to preparation and everything to fortune.
Wasps scored a late try through Ben Jacobs but it was barely any consolation for the hosts, who started the day as favourites to claim the final play-off spot but now find themselves staring at a premature end to their season in a fortnight's time.
And Huddersfield are again going well this time round in fourth, just four points off top spot.
Villa are third in the league - only five points off top spot and seven points clear of fifth place.
Bath went down 20-9 at Vicarage Road and are now 13 points off fourth spot, although they do have games in hand.
Stimson marked a year at the club with a 1-0 win at Macclesfield, to leave the Gills four points off top spot.
Having lost 2-1 to leaders Gabon in their opening match, Morocco have now dropped five points at home and find themselves four points off top spot, having played one game more.
Shropshire skipper James Ralph top scored with 47 for Shifnal but Andy Miller's (5-35) for Walsall gave the Gorway Road side the maximum points, leaving Shifnal just 14 points off bottom spot.
Crusaders had Richard Moore sin-binned at the death, but that did nothing to dampen the euphoria of a win that, temporarily at least, lifts them from the foot of the Super League table, while Huddersfield were knocked off top spot by Warrington's 60-0 thrashing of Salford.
Also an issue: Nationals Park sits in a lonely industrial spot off the freeway.
English rookie Matthew Wilson is off the bottom spot but still remains 52 minutes behind Frenchman Loeb.
BBC: Marcus Gronholm had won four of the six stages on day one
Divers brought it to the surface and then took it to a spot off Kent, during a "delicate" seven-hour operation.
应用推荐