Meanwhile, Ancelotti played down speculation linking Chelsea with a move for Tottenham midfielder Luka Modric.
BBC: Chelsea squad weaker than previous seasons - John Terry
The influential Modric made it two with a powerful 20-yard shot on the turn.
Luka Modric's angled drive doubled the lead, but Matthew Etherington's superb solo goal narrowed the deficit.
And Croatian Football Federation president Vlatko Markovic thinks the timing of Modric's injury is no coincidence.
Substitute Bent, on for Pavlyuchenko, raced onto Modric's through ball but saw his shot saved by Jusskelainan.
Within moments, Modric's blast tied the game and just three minutes later, Real was in front through Cristiano Ronaldo.
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Facing 10 men, Real Madrid coach Jose Mourinho doubled down on attack and sent on the Croatian playmaker Luka Modric.
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Luka Modric, so influential in recent matches under Redknapp had one weaving run but his shot was way off target.
O'Hara was similarly wayward when he skewed his effort after Luka Modric had sent a low free-kick into the box.
The early signs were that the momentum remained with Harry Redknapp's men as long-range shots from Defoe and Modric faded wide.
Meanwhile, Arsenal's north London rivals Tottenham have suffered a setback with a serious injury to Croatia playmaker Luka Modric.
Tottenham looked to make their man advantage count and almost immediately went further ahead, but Luka Modric shot straight at Jaaskelainen.
Rustu was again called into action as Modric crossed for Olic, but it was the last significant action in regulation play.
Despite picking up three points at Ewood Park, Spurs suffered the last-minute loss of key midfielders Steven Pienaar and Luka Modric.
Gold said the suggestion that Lee Bowyer's challenge on Modric was part of a conspiracy was "so ridiculous it beggars belief".
"X-rays have determined that Luka Modric sustained a fracture to his right fibula, " said a statement on the official Spurs Web site.
"Modric is a good player but we are not interested, " he said.
BBC: Chelsea squad weaker than previous seasons - John Terry
Luka Modric's attempted pass to Robbie Keane came to him off Sebastien Bassong and he found the net at the second attempt.
Modric limped out of the game in the 49th minute complaining of a sore calf following an innocuous looking collision with Bowyer.
Last year Arsenal striker Eduardo broke his leg against Birmingham and on Saturday Tottenham's Croatian playmaker Luka Modric suffered the same fate.
They were almost caught out, though, when Spurs broke and Luka Modric crossed for Roman Pavlyuchenko, who should have got his header on target.
However, midfielder Luka Modric and defender Marcelo are in doubt for the first leg of the semifinal after both were taken off with injuries.
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The visitors had early moments of promise but Gareth Bale chested down Luka Modric's sublime crossfield pass to volley Spurs ahead from 10 yards.
Tottenham brought on Darren Bent for Roman Pavlyuchenko and Aaron Lennon for a disappointing Modric, but there was barely any improvement in their attack.
Mourinho also brought on striker Karim Benzema and Mesut Ozil for Higuain and Luka Modric, but Real's best chance was ruled out for offside.
Luka Modric spun clear down the right and crossed to the far post, where Ivan Rakitic's firm header was brilliantly parried by the alert keeper.
The goal briefly unsettled Spurs but they were given a lifeline when a Luka Modric pass wide struck Lampard and played Bent through on goal.
He is a good player but nobody speaks of Modric here.
BBC: Chelsea squad weaker than previous seasons - John Terry
After the upcoming international break, Spurs face a home game against champions Manchester United and a trip to Chelsea, both of which Modric will miss.
At the other end, Vedran Corluka and Modric combined to tee up Defoe but the England striker was denied from close range by the excellent Green.
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