James won men's 100m butterfly silver and Simmonds was pipped to women's 200m backstroke gold, as was Spofforth over the 50m backstroke distance - a race in which Wales' Georgia Davies tied for bronze.
Viewers are being asked to vote on their favourite moments, such as Mo Farah's gold medal wins, Ellie Simmonds' swimming triumphs and even the Queen's James Bond-style skydive.