Today doctors must follow complicated steps to prepare samples, and 10% of DNA material is lost, ruining results.
Police said their cold case review of the death may be helped by the recovery of DNA material from the body which is buried at Malton Cemetery.
Prosecutor Manuela Comodi refuted the testimony from independent forensics experts that cast doubt on the reliability of the evidence, insisting police forensic officers had handled the DNA material properly.
More thorough testing was carried out - including the DNA testing of raw material at Comigel's factory - and the results were confirmed on Wednesday.
They managed to extract the type of DNA found in the nuclei of cells (nuclear DNA) and genetic material from the cell's "powerhouses" - known as mitochondria.
It follows the discovery of horse DNA in burgers imported as a raw material from Poland by the firm.
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In its design we took care that the test can cope with the challenges of forensic DNA analysis such as low amounts of material.
The investigation was given a kick-start last autumn when new technology allowed a DNA profile to be built up from material found on Pauline's clothing.
This vast majority of genetic material known as functional noncoding DNA, mostly embedded within and around the genes, was thought to play an important but murky role in regulating how the coding genes go about their business.
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It focused narrowly on areas in and around active genes, ignoring the 98.5% of genomic material known as "junk DNA." This process pinpointed 25, 000 genetic landmarks called SNPS (single nucleotide polymorphisms, or "snips"), places where a single dna letter varies from one person to the next.
What comes out is a mess of data, strings of genetic material where it is not always easy to tell what DNA comes from what microbe.
Other companies are also in the business of making DNA for drug companies and other research organizations, which save time by using newer DNA synthesis methods instead of laboriously copying cells and inserting or deleting bits of genetic material.
Franco Rollo, at the University of Camerino, and colleagues examined the DNA from the contents of the intestines, some of which represented the trace remains of food, but also other material, such as fungi, which invaded the body after death.
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