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"I stumbled, my legs gave out on me and I got over-excited, " said Demus.
BBC: France's Christine Arron won the 100m in Oslo
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Poland's Anna Jesien was second in 54.43 with Demus crossing the line in 54.59.
BBC: France's Christine Arron won the 100m in Oslo
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The Jamaican ran 52.42 seconds to power home ahead of Lashinda Demus of the United States.
BBC: Walker storms to 400m hurdle gold
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The double-medal performance in three events made Lashinda Demus' silver in the 400-meter hurdles seem like a disappointment.
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In the women's 400m hurdles, Demus stumbled to hand victory to her countrywoman Sandra Glover in a season's best time of 53.93secs.
BBC: France's Christine Arron won the 100m in Oslo
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Commonwealth silver medallist Tasha Danvers-Smith was the best of the Brits as she came home fifth behind race-winner and world number one Lashinda Demus in the 400m hurdles.
BBC: SPORT | Athletics | Powell maintains unbeaten stretch
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Earlier, Walker had started the 400m hurdles quietly but she was on Demus' shoulder at the bend before making her move on the home stretch to claim a comprehensive win.
BBC: Walker storms to 400m hurdle gold
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Demus crossed in 52.77, and sunk to her knees.
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Demus, 29 years old, is the reigning world champion.
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Demus, the 2005 world silver medallist who missed the 2007 worlds for the birth of her twin sons, just managed to stave off the late challenge of Lucas and admitted she was to blame for her defeat.
BBC: Walker storms to 400m hurdle gold
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"I wasn't upset, just disappointed, " Demus said.
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