Matfield was one of several world-class players originally left behind to rest in South Africa, including back-row Schalk Burger and wing Bryan Habana.
Woodward will be looking particularly closely at full-back Dan Scarbrough, wing three-quarters Mark Cueto and Phil Christophers, along with the back-row forwards.
The blunt approach has been underlined by Gatland's candid responses to questions about dropping wing Mark Jones, centre Sonny Parker and back-row replacement Alix Popham.
Louise Rickard, with 110 caps behind her at wing and full-back, is on the bench as second row cover while uncapped number eight Vici Owens and centre Adi Taviner make their first appearance on the bench.