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It leaves Laurent Koscielny and Sebastian Squillaci as the Gunners' only fit senior central defenders, with Thomas Vermaelen still out because of an Achilles tendon problem.
BBC: Arsenal insist Johan Djourou will miss rest of season
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England took a calculated gamble by recalling Pietersen, who has been troubled by an Achilles tendon problem, and he admitted afterwards that he was only "about 70% fit".
BBC: Collingwood hails England display
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The team will be again be led by Ricky Ponting, who missed the recent series against Bangladesh after undergoing a wrist operation and opener Matthew Hayden has been included despite a persistent Achilles tendon problem.
BBC: Australia gamble on spin rookies
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Bolt has been nursing a minor Achilles tendon problem and missed last week's Diamond League meeting in New York, but Mills said that injury was not the reason for his absence from next week's Jamaican trials.
BBC: Usain Bolt to miss Commonwealth Games, says coach
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Two players Flores no doubt hopes do not feature on Wednesday are Fulham striker Bobby Zamora, who faces a race against time to overcome an Achilles tendon problem, and winger Duff, who is recovering from a leg injury.
BBC: Fulham manager Roy Hodgson eyes Europa League glory