Former Great Britain rugby league captain Farrell will miss the match after picking up a calf strain, while he has been joined on the sidelines by Barkley after the Bath man picked up a dead leg during training.
He played for 70 minutes of atraining ground friendly with Grays Athletic on Tuesday, his first game since injuring his leg against Birmingham in August.
Rooney is currently sidelined with a groin injury picked up in training, after having overcome ankle ligament damage sustained during the first leg of the Champions League quarter-final against Bayern Munich.