目的扩增木瓜凝乳酶基因，构建重组表达载体。
PurposeThe chymopapain gene was amplified, and the recombinant vector was constructed.
目的：构建抗腮腺炎病毒抗体轻链基因重组表达载体。
Objective: To construct the recombinant expression vector of genes encoding light chain of antibody against Mumps Viruses.
所述重组表达载体包含有本发明的任何一种DNA序列。
The recombinant expression vector contains any DNA sequence of the invention.
所述表达菌包含有本发明的任何一种重组表达载体或本发明任何一种DNA序列。
The expression fungus contains any recombinant expression vector or any DNA sequence of the invention.
结论：经酶切鉴定构建的人透明带蛋白3重组表达载体可高效表达重组人zp3蛋白。
Conclusion The recombinant expression plasmid constructed by restriction enzyme cleave identification can highly express recombinant human ZP3 protein.
目的：构建融合重组表达载体并对其进行诱导表达和蛋白纯化以获得大量重组融合蛋白。
AIM: to construct the recombinant expression vector and to obtain large amount of mouse Cyclin D1 protein fused to PTD.
结论重组表达载体PGL3 -DF 3 - DTA能对DF 3阳性的乳腺癌细胞产生特异性杀伤作用。
Conclusion the recombinant expression vector PGL3-DF3-DTA can produce a specific killing effect on the DF3-positive human breast cancer cell line.
结论：重组表达载体PGL3 - DF 3 - DTA能对DF 3阳性的乳腺癌细胞产生特异性杀伤作用。
Conclusion: Recombinant expression vector PGL3-DF3-DTA could produce specific lethal effect on human breast cancer cell line of DF3 positive.
目的：构建高效表达人透明带蛋白3的真核重组表达载体。方法：利用PCR、T- A载体克隆和亚克隆等技术。
Objective To construct the recombinant plasmid that highly expressed human zona pellucida 3. Methods The techniques of PCR amplification, T-A vector ligation, and sub-clone were used.
完成了NKG2D的原核表达载体的构建，表达并纯化了重组NKG2D蛋白。
The NKG2D prokaryotic expression vector was successfully constructed. The recombinant NKG2D is expressed and purified.
目的：构建含人IL -21基因的重组腺病毒表达载体，为IL - 21基因治疗肿瘤研究奠定基础。
Objective: to be as the foundation of the tumor therapy with targeting IL-21 gene by constructing of recombinant interleukin 21 gene adenovirus expression vector.
结论构建了含人pd - 1基因重组逆转录病毒的载体，筛选出稳定表达人PD - 1分子的L929细胞株。
Conclusion HumanPD-1 gene has been cloned, recombinant retrovirus vector containing PD-1 gene constructed and L929 transfection cell line expressing PD-1 molecules selected.
目的利用杆状病毒表达载体系统制备重组乙型肝炎病毒核心蛋白。
Objective To produce recombinant HBcAg in insect cells by Baculovirus expression vector system.
本发明还涉及用于在宿主中表达这些功能性结合蛋白的重组载体。
The present invention further relates to recombinant vectors useful for the expression of these functional binding proteins in a host.
实验结果表明，以TK基因构建的重组载体质粒可用于外源基因的重组表达研究。
The result showed that the transfer vector plasmid constructed with FPVTK genes can be used to express foreign gene in FPV recombinant.
本发明还涉及包含所述非病毒载体和所述表达型重组体的药物组合物及其应用。
The invention also relates to the pharmaceutical compositions containing the non-viral vectors and the expression type recombinant and their use.
目的构建人高迁移率族蛋白1（HMGB1）编码基因的表达载体，获得纯化的重组蛋白，研究其生物学功能。
Objective To construct the recombinant vector, the expression of human HMGB1 code gene and to study its function.
结果：成功地构建了重组BMP7逆转录病毒表达载体，并可在骨髓基质干细胞中表达BMP 7。
The viruses were used to infect directly BMSCs and the expression of BMP 7 gene in BMSCs was analyzed by immunohistochemical staining.
目的构建重组人瘦素哺乳细胞表达载体并在COS 7细胞表达重组人瘦素。
Objective to construct recombinant human leptin mammalian cell expression vector, and to express recombinant human leptin in COS-7 cells.
目的：建立一种在甲胎蛋白(afp)阳性的肝癌细胞中靶向表达目的基因的重组腺病毒载体。
AIM: to construct a recombinant adenovirus vector carrying AFP promoter to specifically express a targeting gene in hepatocellular carcinoma HepG2 cells.
结论构建了人瘦素的哺乳动物细胞表达载体，并成功地在COS 7细胞中获得重组人瘦素的分泌表达。
Conclusion Cloning and expression recombinants of human leptin are obtained and secretive recombinant human leptin is successfully expressed in COS-7 cells.
本发明公开了一种食蟹猴P 4502e1药物代谢酶及其与食蟹猴p450氧化还原酶的共表达的重组载体。
The invention discloses a recombinant vector co-expressed by medicament metabolizing enzyme P450 2e1 of cynomolgus monkey and REDOX enzyme P450 of cynomolgus monkey.
构建GGT1重组真核表达载体，观察GGT1在COS7细胞中的定位。
To construct the eukaryotic expression vector of GGT1 and detect its localization in COS7 cells.
结论：原核表达载体的构建、重组蛋白的表达、纯化及多克隆抗体的制备为今后研究该蛋白的功能提供了良好的基础。
CONCLUSION: Recombined prokaryotic expression vector, the purified protein and prepared polyclonal antibody were the necessary materials for further study of this protein.
目的构建人鸟氨酸脱羧酶抗酶(OAZ)突变基因，重组至原核表达载体中并表达出重组蛋白。
Objective To clone human ornithine decarboxylase antizyme (OAZ) mutation gene and express its recombinant protein.
目的构建人鸟氨酸脱羧酶抗酶(OAZ)突变基因，重组至原核表达载体中并表达出重组蛋白。
Objective To clone human ornithine decarboxylase antizyme (OAZ) mutation gene and express its recombinant protein.
应用推荐