目前，评价土壤中金属植物有效性的方法主要有：化学提取法、植物吸收试验、微生物和酶活性试验及同位素法。
The main methods now to evaluate Pb availability in soil are chemical extraction, plant adsorption, microbial and enzyme activation and isotopes tests.
本方法提取成本低，操作简便，酶活回收率高，因此具有应用价值。
The method possesses the practical value for its advantages of low production cost, simple operation and high enzyme recovery.
方法提取人外周血单个核细胞总RNA，反转录聚合酶链反应（RT-PCR）扩增目的基因；
Methods RNA from human peripheral blood mononuclear cells were extracted and target genes were amplified by RT-PCR.
本发明公开了一种采用超高压调控蒜氨酸酶反应提取洋葱精油的方法，属于农产品精深加工技术。
The invention discloses a method for extracting onion essential oil through ultrahigh voltage regulation of alliinase reaction and belongs to fine and deep processing technology for farm products.
方法提取肿瘤组织总rna，用反转录聚合酶链式反应(RT - pcr)法进行检测。
Methods Total RNAs of tumor tissues were extracted and were detected by RT-PCR.
主要研究了麦芽根中5′—磷酸二酯酶的提取方法。
The methods of 5 '-phosphodiesterase extraction from malt root were studied in this paper.
主要研究了麦芽根中5'磷酸二酯酶的提取方法。
The methods of 5 '-phosphodiesterase extraction from malt root were studied in this paper.
用微波和高压提取的方法提取的果胶低聚糖比用超滤膜酶反应器的提取的具有更高的聚合度。
Pectic oligosaccharide produced by microwave and autoclave extraction were observed with higher degrees of polymerization than those by an ultrafiltration membrane enzyme reactor.
方法：采用多重聚合酶链反应方法，以提取的基因组d NA为模板，在同一反应管中同时扩增细胞周期素d 1基因和P 16基因。
METHODS: Method of multiplex polymerase chain reaction (PCR) was adopted. Cyclin D1 gene and P16 gene were amplified in the same tube, using extracted genome DNA as template.
方法：采用正交试验，优化玉米须多糖的酶及超声提取方案，并研究这两种方案的结合效果。
Methods: Enzyme and ultrasound-assisted extraction conditions were optimized respectively through orthogonal test. A combination of the two methods was investigated.
以蛋壳膜为原料，采用稀碱与酶解相结合的方法提取硫酸软骨素。
Chondroitin sulfate was extracted from the eggshell membrane with a method of dilute alkali-enzyme hydrolysis.
番茄红素的提取方法主要包括：有机溶剂提取法、酶反应法、超临界CO2萃取法等。
The extracted methods of lycopene mainly include: organic solvent extraction, enzymatic reaction extraction and supercritical CO2 extraction.
方法：可溶性胶原蛋白从小牛皮中提取，用胃蛋白酶处理而去除其抗原性。
METHODS: Soluble collagen was extracted from calf fetus skin and was made nonantigenic with pepsin.
采用现代生物酶技术降解的有关原理和方法提取米糠的主要营养物质，对提取米糠营养素生产工艺、工艺参数进行研究并在食品生产中应用。
Technology of biological fermentation was used to extract the main nutrient in rice bran, the process and parameters were researched. This method is used in food production.
以蛋白酶K消化、苯酚及氯仿抽提以及异丙醇沉淀的方法提取全基因组DNA。
DNA was extracted by proteinase K digestion, phenol and chloroform extraction as well as isopropanol precipitation.
主要研究了麦芽根中核酸酶的提取方法。
The methods of nuclease extraction from malt root were studied in this paper.
采用超声和差速离心方法，研究了猪肝线粒体ATP合酶水溶性部分（F1-ATP合酶）的提取及检测方法。
The extraction and detection of F1-ATP synthase from pig-liver mitochondria are investegated by using ultrasonic and differential centrifugal methods.
对从大蒜中提取超氧化物歧化酶(SOD)进行了探索性实验。通过不同的方法提取、纯化SOD，从而找到活性高、纯化倍数高的较好方法。
Extraction and purity of superoxide dismutase (SOD) by different method are demonstrated, and a good method with high activity and purification multiple has been found.
本发明公开了一种蜈蚣提取的有效部位，还公开了这种有效部位的制备方法，它是蜈蚣通过仿生酶解法制得。
The invention discloses an effective part extracted from a centipede and a method for preparing the effective part. The effective part is prepared from the centipede by a bionic enzymolysis method.
方法分离猪血细胞，用溶血，脱水，干燥等方法提取鞘磷脂，并用磷脂酶C降解鞘磷脂成神经酰胺。
METHOD Sphingomyelin was prepared through the process of separating erythrocyte from blood, erythrocytolysis, dehydration, dry, et al, and then hydrolyzed to ceramide by phospholipase C.
利用对油籽细胞壁的机械破碎和酶降解的方法，建立起一种玉米胚油提取新工艺。
A new procedure for obtaining corn germ oil was developed using mechanical and enzymatic rupturing of cell walls.
方法：采用改良CTAB法从忍冬叶片中提取DNA，通过琼脂糖凝胶电泳、紫外分光光度法、酶切检测其质量，并与常规CTAB法比较。
Method:Two different methods, conventional CTAB method and modified CTAB method, were applied to extract genomic DNA from leaves of Lonicera japonica Thunb.
本文研究了多种植物种子中植物酯酶的提取方法和总酯酶活力，得出了最佳提取条件。
The extraction methods for optimum conditions and the general esterase activity of phytoesterase were studied.
介绍了采用碱处理法、酸处理法、氧化法、酶处理法等从皮革废弃物中提取胶原蛋白的几种方法，还介绍了这些胶原蛋白在造纸中的应用现状及发展趋势。
The extractions of collagen from tannery wastes by acid, alkali, enzyme, oxidation etc were introduced. The application of collagen fiber in papermaking and its developing tendency were also analyzed.
重组酶具有选择特异性更高的特点，与生化提取方法相比成本较低廉。
The recombinant enzyme has several advantages, such as the better catalyzed ability and fewer producing expenses.
酚-氯仿法从外周血中提取基因组dna，聚合酶链反应(PCR)及限制性片段长度多态性(RFLP)方法检测CYP11 B2基因C - 344t多态性。
Genome DNA was extracted from white blood cell. Polymerase chain reaction (PCR) and restriction fragment-length polymorphism (RFLP) were employed to study C-344T polymorphism of CYP11B2 gene.
从土壤的选择性培养液中提取微生物的DNA，通过PCR的方法直接扩增成功得到GL-7-ACA酰化酶基因。
The genomic DNA was extracted from the selective cultures and the GL-7-ACA acylase gene was directly amplified by PCR technique.
研究了胡萝卜多糖的超声波协同酶法提取的方法。
The optimized parameters of carrot polysaccharide extraction by the cellulase enzymatic hydrolysis with ultrasonic technologies were obtained.
方法采用酶直接消化法从胎盘组织中提取间充质干细胞，并利用流式细胞术及染色体核型分析技术鉴定其干细胞特性。
Methods the enzyme directory digestion method was used to isolate Mesenchymal stem cells from Human Placenta and flow cytometry and karyotyping were used to evaluated the character of stem cells.
方法采用酶直接消化法从胎盘组织中提取间充质干细胞，并利用流式细胞术及染色体核型分析技术鉴定其干细胞特性。
Methods the enzyme directory digestion method was used to isolate Mesenchymal stem cells from Human Placenta and flow cytometry and karyotyping were used to evaluated the character of stem cells.
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