目的:建立蒜酶催化蒜氨酸反应产物的HPLC-MS分析方法。
Objective: To develop HPLC-MS method for detecting catalytic reaction products of alliin by alliinase.
方法:使用电喷雾电离源分析蒜酶催化蒜氨酸反应产物。
Methods: HPLC-MS equipped with an electro spray ionization (ESI) source was used for this study.
综述了酶催化立体选择性合成及动力学折分制备旋光性氰醇的几种方法。
Several enzymatic methods, including enzyme catalyzed enantioselective synthesis and kinetic resolution, for the preparation of optically active cyanohydrins are reviewed.
与以传统方法制得的类似的酯相比,由象脂肪酶这样的酶催化合成的酯的气味要好得多。
It was also observed that fatty acid esters, synthesized by enzymes such as lipase, often have better odour and flavour characteristics compared to similar esters produced by conventional means.
本文对酶催化剂的固定化方法以及在有机催化反应中的应用作了部分简述。
The immobilization methods of enzyme catalyst and the applications in catalysis reaction were introduced in this paper.
生物可降解聚酯是一种新型高分子聚合材料,可通过发酵、化学方法和酶催化来合成。
Biodegradable polyesters are polymer materials, they can be synthesized by chemical methods, enzymatic-catalysis and ferment.
利用脂肪酶催化油脂水解成脂肪酸和甘油,从而得到脂肪酸产品是一种温和的油脂水解方法。
It's a mild hydrolysis method to get fatty acid product that use lipase as a catalyst to hydrolyze oil into fatty acid and glycerol.
提出了一种基于酶催化沉积放大的电化学免疫分析方法。
A novel electrochemical immunoassay method based on enzyme-catalyzed depositing enlargement is proposed.
DNA双螺旋链在复制和转录的过程中要在酶的催化下解旋,这是一个高度复杂的过程,目前还无法用物理学的方法来描述。
During the replication and transcription, DNA unwinds the double helix under the control of an enzyme. It is a very complex process beyond a physical description.
利用反应动力学方法,探讨了含有多种中间复合物的酶催化反应动力学模型。
Taking advantage of kinetic method, we discussed the kinetic model Of multiple substrat enzymatic reaction.
以啤酒酵母为酶源,催化—磷酸腺苷(AMP)生成三磷酸腺苷(ATP),是最有工业前景的ATP生产方法之一。
The production of adenosine triphosphate(ATP) from adenosine monophosphate (AMP) catalyzed by beer yeast is regarded as one of the most promising techniques in ATP industry.
血清醌还原酶(QR)、ALT、LDH和CK催化活性用酶动力学方法测定,肝丙二醛(MDA)含量按硫代巴比妥酸方法测定。
Serum quinone reductase (QR), ALT, LDH and CK activities were determined with enzyme dynamic methods. Liver malondialdehyde (MDA) content was measured by the method of thiobarbituric acid.
目前一种新的研究动向是将两者结合起来用于催化合成反应,这种新型催化方法可以被称作微波辐射-酶耦合催化(MIECC)。
A new trend in organic catalyzing synthesis is to utilize these two strategies simultaneously, which is called microwave irradiation-enzyme coupling catalysis (MIECC).
重点阐述了氯代苯甲酸的一系列合成方法,并简要介绍了金属卟啉模拟酶仿生催化氧气氧化氯代甲苯绿色合成氯代苯甲酸的新方法。
And gave a brief introduction of a novel technology for green synthesis of chlorobenzoic acids by dioxygen oxidation of chlorotoluenes using metalloporphyrins as biomimetic-catalysis.
本发明涉及一种催化大分子底物反应的交联酶聚集体及其制备方法。
The invention relates to cross-linked enzyme aggregate for catalyzing reaction of macromolecule substrate and a preparation method thereof.
与传统的化学合成法相比,脂肪酶催化合成内酯具有方法简单、反应条件温和和产率高等特点。
Compared to conventional chemical synthesis, lipases catalyzed synthesis of lactones is simple, mild and highly productive.
本发明涉及一种新型的二元酸酯的制备方法,包括采用固定 化脂肪酶,由二元脂肪酸和一元脂肪醇催化合成二元酸酯的步骤。
The invention relates to a method for preparing a novel diester, which comprises steps of synthesis using immobilization lipase catalyzed by biprotic fatty acid and monohydric fatty alcohol.
本发明涉及在全细胞催化剂或分离的酶的辅助下从醛生产伯醇的方法。
The invention relates to a method for producing primary alcohols made of aldehydes with the aid of whole cell catalysts or isolated enzymes.
反应可以通过酶催化或非酶催化的手段来实现,也可以通过两种方法的有机结合来进行。
The reaction can be realized by enzymatic or non-enzymatic catalysis or by combination of two methods.
检测一个细胞样品中是否存在催化活性形式或非催化活性形式的甲基腺苷磷酸酶(MTA酶)的方法。
A method for the detecting whether methyladenosine phosphatase (MTAse) is present in a cell sample in either a catalytically active or catalytically inactive form.
在用于实际试样的回收率测定中,结果良好。(2)提出了一种基于酶催化沉积底物和生物素-亲和素两步放大的超灵敏免疫检测方法(第3章)。
An amplified immunoassay with improved sensitivity has been proposed based on two-step amplification procedures of enzyme-catalyzed precipitation and biotin-streptavidin binding system (in Chapter 3).
测定方法产品说明过氧化氢酶(EC1.11.1.6)是一种普遍存在的抗氧化酶,主要作用是催化过氧化氢(H2O2)分解为水和氧气。
Descript ion CATALASE (ec 1.11.1.6), is an ubiquitous antioxidant enzyme that catalyzes the decomposition of hydrogen peroxide (H2O2) to water and oxygen.
方法:测定酶催化反应后底物浓度的变化,计算GST活力单位。
Methods:By detecting the concentration of the substrate after enzyme added the activity of the enzyme in the fluid was calculated.
本论文介绍了酶学研究的进展,酶的催化机制、研究方法和分子酶学、功能酶学及应用酶学的研究现状。
This thesis investigates the progress of the enzyme, the enzyme's catalytic mechanism, research methods and molecular enzymology, functions and application of enzymology.
本发明公开了一种修复土壤ddt污染的方法,将漆酶投加到DDT污染的土壤中直接催化降解残留在土壤中的DDT。
The invention discloses a method for renovating soil polluted by DDT. Laccase is added into the soil polluted by DDT for directly catalyzing and deteriorating DDT residual in the soil.
本发明公开了一种修复土壤ddt污染的方法,将漆酶投加到DDT污染的土壤中直接催化降解残留在土壤中的DDT。
The invention discloses a method for renovating soil polluted by DDT. Laccase is added into the soil polluted by DDT for directly catalyzing and deteriorating DDT residual in the soil.
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