方法:对临床分离的85株金黄色葡萄球菌,用酚氯仿法提取DNA后进行RAPD分型。
Methods: 85 clinical strains of S. aureus were extracted by phenol chloroform method and the RAPD typing was also performed.
方法对临床分离的15株金黄色葡萄球菌,酚氯仿法提取其DNA后行RAPD分型,同时进行噬菌体分型。
METHODS DNA from 15 clinical strains of S. aureus were extracted by phenol-chloroform method and used in the RAPD assay, phage typing were also performed.
方法使用碱裂解法、酚氯仿法和煮沸裂解法同时提取血清hbv - DNA,比较荧光定量pcr测定的重复性和测定值的差异。
Methods an Alkaline lysis method was used to isolate serum HBV-DNA for fluorescence quantitative PCR and compared with conventional phenol-chloroform extraction method and boiling lysis method.
目的建立运用酚-氯仿法结合磁珠法从蝇蛆嗉囊内提取人类DNA的方法,从而提高STR分型检验的灵敏度。
Objective To establish an effective phenol-chloroform method coupled with paramagnetic particle method for human DNA extraction from maggot crop contents in STR genotyping.
酚-氯仿法从外周血中提取基因组dna,聚合酶链反应(PCR)及限制性片段长度多态性(RFLP)方法检测CYP11 B2基因C - 344t多态性。
Genome DNA was extracted from white blood cell. Polymerase chain reaction (PCR) and restriction fragment-length polymorphism (RFLP) were employed to study C-344T polymorphism of CYP11B2 gene.
方法:应用本层析柱方法从金鱼组织所提取RNA和采用异硫氰酸胍-酚-氯仿一步法提取RNA的比较。
Methods: To compare the method of the extract of RNA from goldfish using laminar analysis column with that of using one-step guanidinium isothiocyanate-alcohol phenyl-chloroform.
方法:应用本层析柱方法从金鱼组织所提取RNA和采用异硫氰酸胍-酚-氯仿一步法提取RNA的比较。
Methods: To compare the method of the extract of RNA from goldfish using laminar analysis column with that of using one-step guanidinium isothiocyanate-alcohol phenyl-chloroform.
应用推荐