利用通用引物（ITS1和ITS4）扩增、克隆和测序了大孢指疫霉ITS区段。
The internal transcribed spacer (ITS) region was amplified with the general primer (ITS1 and ITS4).
利用通用引物成功扩增了黄鳍鲷和黑鲷的线粒体细胞色素氧化酶i亚基(COI)基因序列。
Mitochondrial cytochrome oxidase subunit I (COI) fragments of Sparus macrocephalus and Sparus latus were amplified using universal primers.
经设计通用引物、PCR扩增、克隆和测序，首次从分子水平鉴定了杂草赛葵上的双生病毒。
We identified the geminivirus in Malvastrum coromandelianum from the molecular level by designing the primers, PCR, cloning and sequencing.
方法以一段特异性通用引物并配合热启动聚合酶链反应技术来检测临床标本中的棘阿米巴原虫。
Methods We used a hot initiated PCR based method with asset of universal acanthamoeba specific primers to detect acanthamoeba.
新方法扩展了“通用引物”的适用范围，并为引物设计和一些其它基因的PCR放大提供了思路。
Our method extended the utility of universal primers and provided implications for primer design and PCR amplification of some other genes.
目的：应用内转录间隔区(its)通用引物建立一种较为快速、简便的检测和鉴定念珠菌的聚合酶链反应(PCR)方法。
Objective: To set up a rapid and simple PCR assay for Candida detection and identification by using internal tran scribed spacer (ITS) universal primers.
结论采用通用性强的引物系统配合特异性高的热启动PCR技术检测临床和实验室标本中是否存在病原性真菌的方法有重要的应用潜力。
Conclusion This highly universal primer system in combinaition with highly specific hot initiated PCR might be used in the detection of medically important fungi in experimental or clinical specimens.
利用SSR引物的种间通用性，本研究分别从杨属和柳属中各随机筛选出10对SSR引物，对17个杨树品种和20个柳树品种的遗传关系进行了分析。
In this research, random screening of 10 SSR primers from Populus and Salix, respectively, and the genetic relationship among 17 poplar cultivars and 20 willow cultivars were studied.
利用SSR引物的种间通用性，本研究分别从杨属和柳属中各随机筛选出10对SSR引物，对17个杨树品种和20个柳树品种的遗传关系进行了分析。
In this research, random screening of 10 SSR primers from Populus and Salix, respectively, and the genetic relationship among 17 poplar cultivars and 20 willow cultivars were studied.
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