目的：探讨影响电转染效率的因素。
Objective: To explore the effect of influencing factors on electrotransfection efficiency.
微泡浓度为10％时细胞转染效率最高。
Microbubbles at concentration of 10% led to the highest transfection effect.
培养液中的白蛋白、血清可降低转染效率。
Albumin, serum in the culture medium decreased the transfection efficiency.
然而低转染效率限制了其在临床上的应用。
However, low genetic transfection efficiency limits their clinical application.
流式细胞仪检测转染效率，MTT法检测细胞毒性。
The efficiency of transfection is examined by Flow CytoMeter and the cytotoxicity is evaluated with MTTassay.
其二，研究了基因载体的结构对于基因转染效率的影响。
In the second part, we investigated the influence of gene delivery vectors on gene transfection efficiency.
随着碳链长度的增加，表面活性剂的转染效率随之降低；
The transfection efficiency decrease with the increase of the alkyl chains.
用电泳实验和绿色荧光蛋白标记方法研究了它的转染效率。
We used gel retardation assay experiment and green fluorescence protein marker to investigate their transfer efficiency.
结论可以利用阳离子脂质体来提高重组腺病毒的转染效率。
CONCLUSION Lipofectamine may be used as a tool to enhance the efficacy of transfection with recombinant adenovirus.
目的尝试利用腺病毒介导慢病毒载体的细胞感染以提高转染效率。
Purpose To test the hypothesis that adenovirus may improve gene transfer efficiency of lentiviral vector by mediating viral entry.
然而，转染效率的增加，使得人们对基因整合等安全性问题的担心更甚。
Nevertheless, with the increasing of transfection rate, worried about safety problems such as gene integration were worse.
对原始壳聚糖载体进行改性，提高其转染效率和对于呼吸道细胞的靶向性。
The original chitosan carrier is modified to improve the transfection efficiency and the targeted property to respiratory cells.
在标准转染条件下，增加DNA用量和延长细胞暴露时间反而使转染效率下降。
Under standard transfection conditions, increasing DNA amount and exposure time of the DNA-liposome complexes with cells resulted in decreased transgene expression.
本发明所要解决的技术问题是提供一种转染效率高且具有肿瘤血管靶向性的阳离子脂质体。
The invention aims at solving the technical problem of proving a cationic liposome with high transfection efficiency and tumor vascular targeting.
已有实验表明，细胞转染效率可能决定于DNA -脂质体复合物的形成以及所转染的细胞种类。
Previous studies have shown that the level of gene expression achieved may be dependent on the formulation of the DNA-liposome complex and the cell type transfected.
这种含有较多DNA和较高N/P比的多层膜为提高原位基因传递体系的转染效率提供了一种新方法。
The film with more DNA and higher N/P ratio might provide a new method to enhance transfection in localized gene delivery system.
腺病毒作为新兴的载体因其转染效率和表达效率高而日益受到重视，成为肿瘤基因治疗的主要载体之一。
As the newly emerging vector, recombinant adenovirus is being given more attention and has become the major vector of gene therapy because of its high rate of transfection and expression.
我们选取了转染效率较高且SEPT 6基础表达量不是很高的非小细胞肺腺癌h 1299作为细胞模型。
We choose Non small cell lung adenocarcinoma cell line H1299 as cell model for its low endogenic SEPT6 expression and high transfection efficiency.
为了提高受体介导的基因转染效率，本文提出一种基于PEI和FOLOPEGPLL共轭物的新型、简便的合成方法。
For efficient receptor-mediated gene transfection, a new and simple formulation method based on using PEI and FOLPEGPLL conjugate was presented.
分别研究了在亲水性云母表面和疏水性PD MS表面细胞图案化的方法，并且对这些图案化细胞的基因转染效率进行了研究。
We developed new methods to pattern cells on both hydrophilic mica surface and hydrophobic PDMS surface and investigated the gene transfection efficiency on the patterned cells.
结论：逆转录病毒介导的HSV -TK系统对人胃癌细胞有较高的转染效率，GCV对转染阳性的胃癌细胞有显著的杀伤作用。
Conclusion: transfection of gastric carcinoma with HSV-TK has higher transfection efficiency. MKN28TK cells are significantly sensitive to GCV.
DOTAP复合物的转染效率明显高于游离AS-ODN；细胞是否贴壁生长及贴壁生长的细胞密度也影响AS-ODN的转染效率。
AS-ODN/DOTAP complex could exhibited more higher transfection efficiency than free AS-ODN and the transfection efficiency was also influenced by many other factors such as cell density etc.
将具有特殊生理功能的多肽作为基因或药物载体、或用于药物修饰等，可以提高基因转染效率，增强药物的靶向治疗效果，降低药物的毒副作用。
Due to the specific bioactivity of peptides, peptide-based gene and drug delivery systems could achieve improved gene transfection efficiency, enhanced drug targeting property and reduced side-effect.
结果纳米颗粒转运系统能够保护被转运的基因，有较高的转染效率，具有良好的靶向性，并且提高了药物的生物利用度，显示出一定的缓控释作用。
Results Nanoparticle carrier system can protect the genes, have a high carrying efficiency and a well targeting property, it also can improve the bioavailability, sustained and controlled release.
方法将携带P16基因的腺病毒体外转染人膀胱癌t 24细胞，观察腺病毒对T24细胞的转染效率以及P 16基因修饰的T24细胞体外生物学特性的改变。
Methods P16 genes were delivered to the T24 cells jn vitro by adenovirus vectors. Gene transfer efficiency and changes of biological specificity of T24 cells were analyzed.
目的比较脂质体介导法和裸质粒直接转染法导入CYP2J3基因表达的效率。
Objective To study the expression efficiency of CYP2J3 gene in rat smooth muscle cells (SMCs) by liposome mediated and naked plasmid transfection.
基因在哺乳动物细胞中的表达效率受到细胞状态的影响，研究图案化细胞的基因转染情况对于基因治疗有着重要的意义。
Gene expression in mammalian cells will be influenced by the cell status. Consequently, gene transfection studies on patterned cells are of great meaning for their gene therapeutic applications.
目的：探讨小鼠心脏移植中，通过在体外保存期间对供心进行灌注，进行重组腺病毒基因转染的方法及效率。
AIM: To establish a method of in vitro donor heart perfusion in murine cardiac transplantation during preservation and apply it in adenovirus mediated gene transfection for donor heart.
结论采用绿色荧光蛋白为报告基因的真核细胞转染技术中，脂质体法是效率高、安全性大的方法。
ConclusionUsing GFP as a report gene in eukaryotic cell transfection, the lipofectamine method could get a higher transfection efficiency and higher survival rate for cells.
结论采用绿色荧光蛋白为报告基因的真核细胞转染技术中，脂质体法是效率高、安全性大的方法。
ConclusionUsing GFP as a report gene in eukaryotic cell transfection, the lipofectamine method could get a higher transfection efficiency and higher survival rate for cells.
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