最近的研究表明脂质体在转染呼吸道上皮细胞中是有用的。
Recent studies have shown lipoplexes to be useful in transfecting respiratory epithelial cells.
逆转录病毒；稳定转染；干细胞。
Retrovirus vector; Stable transfected cell line; Stem cells.
共转染获得重组病毒用于感染家蚕。
培养液中的白蛋白、血清可降低转染效率。
Albumin, serum in the culture medium decreased the transfection efficiency.
然而低转染效率限制了其在临床上的应用。
However, low genetic transfection efficiency limits their clinical application.
转染后的脐血细胞周期生物学特性无异常。
The cell cycle of transferred CBMNC conserved normal biological characteristics.
这一结果在新霉素包含子转染中也得到了证实。
This result was also confirmed by neomycin gene-containing replicons with same mutations.
应用基因转染技术可能是解决这些问题的途径之一。
The gene transfection technology might be one of the approaches to resolve these issues.
目的：研究酪氨酸激酶转染对鼻咽癌细胞成瘤的影响。
Objective To study the effects of transfection of tyrosine kinases on the tumorigenesis of nasopharyngeal carcinoma.
结论反义vegf基因转染可以有效地抑制喉癌的生长。
Conclusions: The growth of tumor can be inhibited significantly by antisense-VEGF gene transfection.
令人担心的是它会转染到那些很容易在病人间传播的细菌中。
The fear would be that it gets into a strain of bacteria that is very good at being transmitted between patients.
结论可以利用阳离子脂质体来提高重组腺病毒的转染效率。
CONCLUSION Lipofectamine may be used as a tool to enhance the efficacy of transfection with recombinant adenovirus.
用电泳实验和绿色荧光蛋白标记方法研究了它的转染效率。
We used gel retardation assay experiment and green fluorescence protein marker to investigate their transfer efficiency.
基因转染2周后行股动脉切除术建立右侧大鼠后肢缺血模型。
After 2 weeks of gene transfection, the femoral arteries of the rats were excised to establish the model of left hind-limb ischemia.
目的以电穿孔法转染COS7细胞系以得到较高的转染率。
Objective To optimize the transfection rate in COS 7 cell line with electroporation method.
此外，PEI也被认为具有高转染率和使内涵体破裂的能力。
Additionally, PEI is considered to exhibit high transfection efficiency and endosomal disrupting capacity.
目的研究阳离子脂质体膜的组分对细胞摄取和基因转染的影响。
OBJECTIVE To study the effect of the lipid compositions on cellular uptake and gene transfection mediated by cationic liposomes.
目的：以CHO - K1细胞为宿主基因瞬时转染条件的优化。
Objective: To optimize the condition of gene transient transfection in CHO-K1 cell.
目的：在中国仓鼠卵巢(CHO)细胞上稳定转染咪唑啉1受体。
Objective: to stably transfect imidazoline-1 receptor (I 1r) in Chinese hamster ovary (CHO) cells.
方法细胞瞬时和稳定转染及氯霉素酰基转移酶和芳香族化酶活性测定。
Methods Transient and stable transfection experiments in cells and assays of chloramphenicol acetyltransferase activity and aromatase activity were used.
检测转染组和未转染组顺铂杀伤率、细胞凋亡和线粒体膜电位的变化。
Using MTT and flow cytometer, we detected the changes of cell mortality rate, apoptosis and mitochondrial membrane potential.
方法：用磷酸钙共沉淀的方法向血管成纤维细胞转染MKP1的基因；
Methods: MKP 1 gene was transfected into vascular fibroblasts by the classical calcium phosphate coprecipitation technique.
目的：研究人ice基因转染联合化疗药物诱导体外杀伤肝癌细胞的作用。
Objective: to investigate the cytotoxicity of ICE gene transfection in Combination with Antitumor Chemicals killing Hepatocellular Carcinoma Cells in vitro.
在标准转染条件下，增加DNA用量和延长细胞暴露时间反而使转染效率下降。
Under standard transfection conditions, increasing DNA amount and exposure time of the DNA-liposome complexes with cells resulted in decreased transgene expression.
目的探讨超声介导脂质体微气泡转染反义寡核苷酸ha- 2741的有效性。
Objective To explore the efficiency of transfection of antisense oligonucleotides HA-2741 by ultrasound mediated liposome microbubbles.
结论体外转染白喉毒素a基因可杀伤人肺腺癌细胞并降低人肺腺癌细胞的致瘤性。
Conclusion: Transfection of DTA gene in vitro may kill human lung adenocarcinoma cells and reduce the oncogenicity of human lung adenocarcinoma cells.
目的：考察不同脂质体材料对阳离子脂质体细胞转染率和脂质体膜流动性的影响。
Objective: To investigate the influences of different lipids on cellular delivery and fluidity of liposome membrane of cationic liposome.
DKK1抗体识别转染水平的人源dkk1蛋白，此抗体与DKK2没有交叉反应。
DKK1 antibody detects transfected levels of DKK1 protein. This antibody does not cross-react with DKK2.
使用该抗体证实，转染293t细胞后表达的IRF - 7主要分布在细胞质内。
We also showed that the IRF-7 was localized mainly in the cytoplasm of transfected 293t cells.
使用该抗体证实，转染293t细胞后表达的IRF - 7主要分布在细胞质内。
We also showed that the IRF-7 was localized mainly in the cytoplasm of transfected 293t cells.
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