方法：基因重组技术及蛋白纯化技术。
本文论述了基因重组融合蛋白纯化过程和存在的问题。
The paper describes process of recombinant fusion protein in purification and existing problems.
其中，已对从牛脑热休克蛋白纯化提出的多克隆抗体，检测人类热休克蛋白质。
Polyclonal antibodies which had been raised against HSPs purified from bovine brains were used to detect human HSPs.
然后诱导表达，用SDS - PAGE及蛋白印迹进行鉴定，然后进行蛋白纯化。
Then it was induced to expression and identified by SDS-PAGE and Western-blotting, and it was purified.
目的：构建融合重组表达载体并对其进行诱导表达和蛋白纯化以获得大量重组融合蛋白。
AIM: to construct the recombinant expression vector and to obtain large amount of mouse Cyclin D1 protein fused to PTD.
藻类养殖及藻红蛋白纯化的高成本已成为藻红蛋白在医药和工业领域广泛应用的重要制约因素。
The cost of mass production of alga and purification of phycoerythrin will seriously constrain the wide range demands of B-phycoerythrin applications in biomedical and food industries.
实验结果表明MALDI-TOF-MS法是检测蛋白纯化过程和分析蛋白相对分子质量十分有效的手段。
The results indicate that MALSI- TOF- MS is one of the most effective tools for the assessment of protein purification processes and for the determination of protein molecular masses.
这将为进一步研究其体外可控性剪接、构建高效的蛋白纯化系统和深入研究蛋白质内含子的剪接机制提供基础。
This will help us to investigate controllable protein cleavage in vitro, further establish efficient protein purification system and investigate splicing mechanism of split-inteins.
将表达的重组E2蛋白纯化后作为ELISA包被抗原，建立了一种基于重组E2蛋白的间接ELISA方法，并对此方法的反应条件进行了优化。
An indirect ELISA was developed and optimized with the purified recombinant E2 protein as coating antigen. The optimal conditions of ELISA were determined as follows.
该蛋白是从菌核病菌核菌中纯化得到的。
This protein has been purified from Sclerotinia sclerotiorum and the EPSP synthase has been analyzed.
将纯化的在大肠杆菌中表达的鹦鹉热衣原体（Cps）的重组主要外膜蛋白（MOMP）与油佐剂混合。
The purified recombinant major outer-membrane protein (MOMP) of Chlamydia psittaci (Cps) expressed in E. coli was mixed with oil adjuvant.
这病毒外部蛋白然后被提纯并产生几百到几千毫升的纯化病毒蛋白，作为抗原，是疫苗中的活性成分。
The outer proteins of the virus are then purified and the result is several hundred or thousand liters of purified virus protein that is referred to as antigen, the active ingredient in the vaccine.
抗体由蛋白A和肽段亲和层析纯化得到。
Antibodies are purified by protein A and peptide affinity chramotography.
多克隆抗体通过用DYKDDDDK合成肽免疫动物制备。抗体用蛋白a和多肽亲和色谱法纯化。
Polyclonal antibodies are produced by immunizing animals with a synthetic DYKDDDDK peptide. Antibodies are purified by protein a and peptide affinity chromatography.
由于时间所限，拮抗蛋白的纯化、测序工作尚未完成，在以后的时间里，本实验室还将继续这方面的研究。
However, the purification of antimicrobial peptides have not yet been finished, it will be carried on in our laboratory in the future.
这需要很高的蛋白产量和纯化能力。
This would not have been possible without high-throughput protein production and purification capabilities.
多克隆抗体由合成化肽段免疫动物，该合成肽段与YAP人源蛋白序列一致，抗体由蛋白a和肽段亲和层析技术纯化。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the human sequence of YAP. Antibodies are purified by protein a and peptide affinity chromatography.
首先，我们在第11章中知道了关于大肠杆菌表达的蛋白产物的纯化工艺优化和特性研究。
First, we learn in Chapter 11 about process optimization and characterization studies for the purification of an E. coli-expressed protein product.
研究者报道，NRF 2活性及其它的纯化蛋白和酶肿瘤标本中高于正常细胞。
NRF2 activity along with its cleansing proteins and enzymes were higher in tumor samples than normal cells, according to the researchers.
抗体采用蛋白a和肽亲和层析法纯化。
Antibodies are purified by protein a and peptide affinity chromatography.
纯化后的目的蛋白的纯度可达到95%。
The purity of target protein could reach 95% after purification.
目的建立重组类人胶原蛋白分离及纯化工艺。
Objective To develop a method for the purification of recombinant human collagen-like protein.
将有机溶剂抗溶剂与高压气体等电沉淀技术结合起来，提出了一种新的蛋白质纯化方法。
A novel method for protein purification was presented, which was the integration of organic antisolvent and compressed gas isoelectric precipitation.
综述近年动物组织蛋白质的提取及纯化技术的研究进展，以期为进一步研究动物组织蛋白质提供帮助和参考。
The paper summarized the extracting and purifying technology of animal tissue protein in recent years, which provides the reference for the further research of animal tissue protein.
目的克隆人热休克蛋白72 (HSP72)基因，原核表达并纯化蛋白产物，以探讨其生物学功能。
AIM To clone human heat shock protein 72 (HSP72) gene, do prokaryotic expression and purify HSP72 protein for further study.
从细胞萃取液或生物液体中分离酶的方法，基本上就是纯化蛋白质的方法。
The methods employed fOR enzyme isolation from cell extracts OR from biological fluids are essentially those fOR the purification of proteins.
从细胞萃取液或生物液体中分离酶的方法，基本上就是纯化蛋白质的方法。
The methods employed fOR enzyme isolation from cell extracts OR from biological fluids are essentially those fOR the purification of proteins.
应用推荐