用免疫蛋白印迹法检测细胞VEGF蛋白表达。
Western blotting was used to detect protein expression of VEGF.
采用蛋白印迹法测定心肌细胞中热休克蛋白70含量。
The content of heat shock protein 70 in myocardium was detected with western blot method.
采用蛋白印迹和ELISA法对表达产物进行特异性鉴定。
The specificity of the expressed products was proved by the immunoblot (WB) and ELISA.
用肺癌患者及健康对照者的血清作第一抗体进行蛋白印迹检测。
The aliquot of proteins was subjected to SDS-PAGE, followed by Western blot analysis in which individual sera were tested for primary antibody.
运用免疫荧光和蛋白印迹技术检测了5种食管癌细胞系中NGAL的表达。
The expression level of NGAL in 5 esophageal carcinoma cell lines was also examined by immunofluorescence and western blot.
骨形态发生蛋白2与骨桥蛋白检测：采用免疫组织化学染色与蛋白印迹方法。
The changes of BMP2 and osteopontin were detected by immunohistochemistry and Western blotting.
然后诱导表达，用SDS - PAGE及蛋白印迹进行鉴定，然后进行蛋白纯化。
Then it was induced to expression and identified by SDS-PAGE and Western-blotting, and it was purified.
蛋白印迹也显示HO-1蛋白水平增加（P<0.01），但HSP70蛋白表达无明显变化。
Western blotting also revealed an increase in HO-1 protein levels(P<0.01) but no change in HSP70 protein expression.
蛋白印迹实验鉴定转基因植株，ELISA方法测定重组蛋白表达水平，集落形成实验测定重组蛋白活性。
PCR and Western blot were used to select the transgenic tobacco plants. ELISA was used to evaluate the expression level while colony forming assay was used to test its biological activity.
之后，他们又应用金标准试验——蛋白印迹法(Westernblot)检测了229名阳性患者，结果表明快速检测结果中有超过10%为假阳性。
They then checked 229 positive patients with the gold-standard laboratory test, a Western blot. More than 10% of the rapid tests were giving false bad news.
目的建立人血浆因子V（FV）蛋白免疫印迹分析，对遗传性FV缺乏症家系成员血浆FV含量进行分析。
Objective To develope a western blot of human plasma factor V (FV) and conduct immunoblot analyses on riasma FV from a family with hereditary FV deficiency.
结果表明：所获得的重组NP蛋白在ELISA分析中均能与阳性血清和单克隆抗体发生特异性结合，但在免疫印迹分析中仅与阳性血清发生特异性结合。
The results showed recombinant NP protein could bind to both positive serum and MAb with specificity in ELISA, but only bind to positive serum in western blot.
蛋白质印迹法：蛋白质分子从电泳凝胶转移到固相介质，然后用抗体进行免疫检测的技术。
Western blotting --- A technique analogous to Southern blotting, used for detection of proteins, usually by immunological methods.
间接免疫荧光、ELISA和免疫印迹检测证明，含SO.7片段的三种重组杆状病毒均在昆虫细胞中表达出相应的活性蛋白。
By IFA, ELISA and Western blot experiments, it was proved all the recombinant baculoviruses containing SO. 7 segment could express biologically active proteins in insect cells.
作者表示，该论文滥用和重复使用他们发表的其他论文中的蛋白 免疫印迹图片，违背了《细胞科学期刊》的编辑政策，必须撤销。
The misuse and re-use of western blot bands violated the editorial policy of Journal of Cell Science, and so we must retract this article.
蛋白的测定采用蛋白质印迹法。
经体外蛋白质—蛋白质之间的相互作用和蛋白质印迹杂交方法进一步验证了PBP1和BNP23的相互作用；
In vitro the interaction between BNP23 and PBP1 was ascertained by means of the protein-protein interaction and western blotting.
表遗传体系包括DNA甲基化、RNA干涉、基因组印迹和组蛋白密码等多方面。
The system of Epigenetics includes DNA methylation, RNA-interfering, genome imprinting and histone code and so on.
用蛋白质印迹免疫检测技术和免疫细胞化学技术鉴定细胞表型。
Immunocytochemistry and western blotting technology were used to identify the cell phenotype.
作为分析化学中蛋白质含量测定的一种新的方法，该技术可用于蛋白质的固定与印迹，以及功能蛋白质组学等领域。
As a novel method of protein determination in analytic chemistry, this technique may be used in protein immobilization and imprinting, functional proteomics, etc.
采用蛋白质分子量标准物和纯化的重组蛋白质进行半干式蛋白质印迹，探讨半干式电泳印迹的多种因素对印迹效果的影响。
To elucidate the effect of the semi-dry electrophoretic transfer and its influencing factors in Western blot, the protein marker and purified recombination protein were used to analyzed.
肝组织GR采用放射性配体结合分析法测定其最大结合容量以及用免疫印迹法测定其蛋白含量。
Maximal binding volume of glucocorticoid receptor (GR) in hepatic tissue was assayed by radio-ligand binding assay and protein content was assayed by Western blot.
斑点印迹试验证实，在人输卵管内膜提取液及输卵管冲洗液中均有BS-1结合蛋白存在。
The dot blot test also demonstrated that the lectin BS - 1 binding proteins presented in human oviductal flushing fluid and epithelium extract fluid.
采用斑点印迹法观察黑色普氏菌能否与人血红蛋白结合。
Dot blot assay was adopted to observe the binding of hemoglobin by P. nigrescens.
用免疫沉淀和免疫印迹法测定胞浆蛋白酪氨酸磷酸化情况。
By way of immunoprecipitation and immunoblotting, the cytosolic protein tyrosine phosphorylation was examined.
实验采用原位杂交，NADPH -NBT组织化学，RNA斑点印迹及蛋白质斑点印迹技术。
The experiment was performed by using in situ hybridization, NADPH NBT histochemistry, RNA dot blot and protein dot blot techniques.
方法利用免疫细胞化学，蛋白质印迹法，RT -PCR和流式细胞术等方法分析细胞周期和对与细胞周期相关的基因表达进行研究。
Methods the cell cycle and the expression of related genes were analyzed by the methods of immunocytochemistry, protein blotting, RT PCR and flow cytometry.
附加表位对于用免疫印迹、免疫沉淀和免疫染色技术来进行蛋白质的标记和检测很有用。
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
目的：确定蛋白免疫印迹检测大鼠脑组织中P-糖蛋白的最佳实验条件。
Objective: To determine the optimum experiment condition of detecting P—glycoprotein with Western blotting.
目的：确定蛋白免疫印迹检测大鼠脑组织中P-糖蛋白的最佳实验条件。
Objective: To determine the optimum experiment condition of detecting P—glycoprotein with Western blotting.
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