将高致敏患者采用PRA单抗原磁珠检测的抗hla特异性抗体的荧光强度转换为MESF单位。
Fluorescence intensity values of anti-HLA specific antibodies determined by Flow PRA single antigen beads of highly sensitized patients were converted into MESF units.
方法对疑似病例组织标本(皮肤、角膜、脑组织等)运用直接免疫荧光实验法检测狂犬病病毒抗原。
Methods rabies virus antigen was detected for the tissue samples (skin, cornea and brain) from the suspected rabies case by direct fluorescent antibody method (DFA).
目的建立亚马逊利什曼原虫无鞭毛体体外培养方法，并用间接免疫荧光法分析其抗原特异性。
Objective To establish a method for culture of Leishmania amazonensis amastigotes in vitro and identify the antigenic specificity of amastigotes with indirect immunofluorescent assay.
共做标本212，发现与胰酶消化处理标本荧光染色效果较好，能排除非特异性抗原干扰。
In 212 cases examined, it was found that the fluorescence staining effect was better in the specimen treated by trypsin digestion. This method can exclude non-specific antigenic interference.
用间接荧光抗体试验在肾切片上耒发现日本血吸虫抗原。
No schistosome antigen could be found on frozen sections of kidneys by indirect fluorescent test.
方法：用直接免疫荧光法查检鼠肺中的病毒抗原。
Methods: Using direct immunofluorescence assay (DIFA) to detect EHF virus from the lungs of rodents.
应用免疫金银染色技术（IGSS）和间接荧光抗体试验（IFAT）对日本血吸虫、华支睾吸虫和卫氏肺吸虫进行抗原定位的研究。
The antigen location of Schistosoma japonicum, Clonorchis sinensis and Paragonimus westermani were studied by using immunogold-silver staining(IGSS) and indirect fluorescent antibody test(IFAT).
方法用酶联免疫吸附试验对982例乙肝患者血清标志物和乙肝病毒前S2抗原进行检测；并用荧光定量PCR法对其进行HBV-DNA检测。
Methods Hepatitis B serum markers and Pre-S2 antigens were tested by enzyme-linked immunosorbent assay and HBV DNA was detected by fluorescent quantitative-PCR in 982 hepatitis B patients.
方法用流式细胞仪检测缺血缺氧后不同时间点星形胶质细胞细胞周期变化，并用荧光免疫细胞化学技术测定胶质细胞纤维酸性蛋白（GFAP）和增殖细胞核抗原（PCNA ）的表达水平。
Methods We measured the astrocyte cell cycles in different time after ischemia and anoxia by flow cytometry and detected the levels of GFAP and PCNA with fluorescence immunocytochemistry.
方法应用间接免疫荧光(IIF)和ELISA方法检测了205例肾脏疾病患者血清中的ANCA及其靶抗原。
Methods ANCA fluorescent patterns and its target antigens in sera of 205 patients with renal diseases were determined by indirect immunofluorescence (IIF) and ELISA.
收集鼠肺和鼠血，用间接免疫荧光法检测鼠肺中抗原和鼠血中抗体。
Rat blood and lungs were collected for indirect immunofluorescent detection of related antigens and antibodies.
方法：以Hep- 2细胞和猴肝切片为抗原基质，用IIF法检测10 5例SL E患者的抗核抗体，分析抗核抗体的荧光模式。
Methods Take HEP-2 cell and primate liver as antigen substrate, examine the ANA of 105 SLE patient's serum with IIF to analyse the immunofluorescent pattern.
对抗原检测的结果与冰冻切片制备抗原建立的间接荧光抗体法完全一致。
The results of the trypsinization antigen IFA and the frozen section antigen IFA were identical.
本方法用粗制抗原即可得满意结果，而不需特殊设备，如荧光显微镜等，易在基层实验室推广。
This method does not need special equipment such as fluorescent microscope and it's easy manipulation is applicabl…
目的：利用抗脱氧雪腐镰刀烯醇多克隆抗体及其人工抗原，采用时间分辨免疫荧光技术建立间接竞争脱氧雪腐镰刀烯醇时间分辨免疫检测方法（DON-TRFIA）。
Objective:An indirect competitive time-resolved fluoroimmunoassay(TRFIA) was established using anti-DON polyclonal antibody and coating antigen DON-BSA to detect deoxynivalenol in cereal.
最后，对制备出的假病毒颗粒进行真核细胞感染实验，以间接免疫荧光法验证上述抗原的真核表达情况。
Immuno-fluorescence assay was used to confirm the expression of the recombinant proteins after virus infection in BHK21 cells.
最后，对制备出的假病毒颗粒进行真核细胞感染实验，以间接免疫荧光法验证上述抗原的真核表达情况。
Immuno-fluorescence assay was used to confirm the expression of the recombinant proteins after virus infection in BHK21 cells.
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