方法采用细胞贴附式膜片钳技术。
方法采用膜片钳技术内面向外式。
Methods The data was recorded by adopting inside out configuration of patch clamp technique.
方法：细胞贴附式膜片钳技术。
METHODS: Cell-attached configuration of patch clamp technique.
方法采用全细胞膜片钳技术。
方法：采用细胞贴附式的膜片钳技术。
METHODS: The date recording from cell - attached patches of patch - clamp technique.
方法：采用制霉菌素穿孔膜片钳技术。
Methods:Using nystatin perforated patch clamp recording configuration.
方法采用全细胞膜片钳技术。
方法全细胞膜片钳技术。
方法采用全细胞膜片钳技术，进行电流钳制。
Methods The whole-cell patch clamp with current clamp was used.
膜片钳技术已成为细胞电生理学研究的重要手段。
The patch clamp technique has become the main method for cell electrophysiology research.
膜片钳技术已成为分子和细胞学研究的重要手段。
The patch clamp technique has become the main method for the molecule and cell research.
方法：采用全细胞膜片钳技术，记录钠通道电流。
Methods: Whole-cell patch clamp technique was used to determine sodium channel cur rent.
作形态学观察，通过单细胞膜片钳技术记录电活动。
Meynert nucleus was obtained and dissociated to observe their morphology and record electric activity by patch clamp technique.
采用全细胞膜片钳技术，记录动作电位和上述各离子流。
We used current-clamp technique to record action potentials, and voltage-clamp technique to record ionic currents.
膜片钳技术给动脉粥样硬化发病机理研究带来了新的亮点。
The patch clamp technique bring about new light set in studying on mechanism of atherosclerotic development.
膜片钳技术的广泛应用极大推动了人们对离子通道的认识。
The widely using of patch clamp technique has greatly promoted our knowledge of ion channels.
文摘：应用膜片钳技术对晶体细胞的研究始于90年代初。
Abstract: the study of lens cells by patch clamp technique began in the early 90 "s."
结论：膜片钳技术使探讨烟碱受体拮抗药的作用位点成为可能。
CONCLUSION: the patch clamp technique makes it possible to observe the action sites of nicotine receptor antagonists.
方法应用膜片钳技术全细胞构型从原代培养的螺旋神经元获得电流钳记录。
Methods Current-clamp recordings with the use of the whole cell configuration of the patch-clamp technique were made from the primary cultured spiral ganglion neurons.
细胞膜离子单通道信号是皮安级的随机电流，膜片钳技术可以记录这些信号。
The signal of single ion channel is stochastic ionic currents on the order of 1pA, which can be recorded by means of patch clamp technique.
膜片钳技术是一种先进的电生理技术，在生命科学研究中已得到了广泛的应用。
The patch clamp recording techniques is an advanced electrophysiological technique, which has been widely used for research in the life science research field.
用膜片钳技术的全细胞模式测定全细胞钠电流并观察应用特布他林对它的影响。
The sodium currents were detected by patch-clamp technique in whole-cell recording mode.
使用全细胞式记录膜片钳技术，研究链霉素对豚鼠耳蜗外毛细胞离子电流的影响。
The effects of streptomycin on ionic currents in outer hair cells isolated from the cochlea of guinea pig were investigated using a whole-cell recording patch-clamp technique.
用全细胞模式膜片钳技术记录分析血管平滑肌细胞L型电压依赖性钙通道的特性。
The electric physiology feature of L-type calcium channels in the vascular smooth muscle cells was analyzed by patch clamp technique (PCT).
急性分离单个的豚鼠气管平滑肌细胞，以膜片钳技术的内面向外式记录单通道电流。
Single channel currents were recorded by using the patch clamp technique in inside-out configuration.
方法：运用全细胞膜片钳技术记录持续性钠电流，并观察其在缺氧-复氧模型下的变化。
Methods: the whole cell patch clamp technology was used to record this current and study its change under the condition of hypoxia-reoxygenation model.
根据检测目的的不同，微电极主要应用可以分为离子选择微电极检测技术和膜片钳技术。
Because the differences of its application, the microelectrode can be divided into ion-selective microelectrode and patch clamp detecting technique.
方法用急性酶解法获得单个大鼠心室肌细胞，标准的全细胞膜片钳技术记录钠通道电流。
The whole-cell patch-clamp recording technique was used to record the change of sodium channel current influenced by carvedilol.
结论成功建立了一种简单快速的适用于膜片钳技术的大鼠皮层神经元急性分离改良方法。
Conclusion a simple and fast method for acutely isolating rats cortex neurons suitable for patch clamp is successfully found.
方法用急性酶解法获得单个大鼠心室肌细胞，标准的全细胞膜片钳技术记录钠通道电流。
MethodsAfter rat hippocampal or cerebral cortical neurons were isolated by pronase e, the IA currents were recorded by whole-cell patch clamp technique.
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